Medical swab touch spray-mass spectrometry for newborn screening of nicotine and cotinine in meconium

Newborn screening is one of public health concerns designed to screen infants shortly after birth. Prenatal exposure to tobacco smoke such as nicotine has been reported to affect babies. Levels of nicotine and cotinine in meconium were widely used to evaluate the tobacco exposure of foetuses during...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2016-12, Vol.51 (12), p.1237-1242
Main Authors: Yang, Bi-cheng, Wang, Feng, Yang, Xiao, Zou, Wei, Wang, Jia-chun, Zou, Yang, Liu, Fa-ying, Liu, Huai, Huang, Ou-ping
Format: Article
Language:English
Subjects:
Chromatography
Cotinine - analysis
Female
Humans
Infant, Newborn
Infants
Limit of Detection
Linear Models
mass spectrometry
meconium
Meconium - chemistry
medical swabs
Neonatal Screening - methods
newborn screening
Nicotine
Nicotine - analysis
Pregnancy
Reproducibility of Results
Screening
Solvents
Spectrometry
Spectroscopy
Tandem Mass Spectrometry - methods
Touch
touch spray
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Summary:Newborn screening is one of public health concerns designed to screen infants shortly after birth. Prenatal exposure to tobacco smoke such as nicotine has been reported to affect babies. Levels of nicotine and cotinine in meconium were widely used to evaluate the tobacco exposure of foetuses during pregnancy in a polluted environment. In this study, medical swabs were applied by using touch spray‐mass spectrometry (TS‐MS) to collect meconium from newborn infants for detection of nicotine and cotinine. Parameters such as choice of spray solvents, solvent volume and collision energy for screening of nicotine and cotinine were optimized. The limits of detection, reproducibility and matrix effect for analysis of meconium were also investigated. In this study, the levels of nicotine and cotinine in 54 puerpera volunteers were screened by TS‐MS and were validated by using traditional liquid chromatography‐mass spectrometry. These results showed that medical swab TS‐MS would be useful for newborn screening of nicotine and cotinine in meconium with high reproducibility, speed, sensitivity and specificity. The use of disposable medical swabs involves no sample preparation and no chromatographic separation, significantly reducing the cost and time required for screening a large number of clinical sample. Copyright © 2016 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.3892