Voltammetry at a Hanging Mercury Drop Electrode as a Tool for the Study of the Interaction of Double-stranded DNA with Genotoxic 4-Nitrobiphenyl

Differential pulse voltammetry, cyclic voltammetry, alternating current voltammetry, and chronocoulometry at a hanging mercury drop electrode (HMDE) and DNA modified HMDE were used for the study of the interaction of 4‐nitrobiphenyl (4‐NBP) with double‐stranded DNA and for the detection of DNA damag...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2016-11, Vol.28 (11), p.2760-2770
Main Authors: Horakova, Eva, Barek, Jiri, Vyskocil, Vlastimil
Format: Article
Language:English
Subjects:
4-aminobiphenyl
4-nitrobiphenyl
Damage detection
Deoxyribonucleic acid
Diffusion coefficient
DNA
Electrodes
hanging mercury drop electrode
interaction
Mathematical analysis
Rate constants
Reduction
Voltammetry
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Summary:Differential pulse voltammetry, cyclic voltammetry, alternating current voltammetry, and chronocoulometry at a hanging mercury drop electrode (HMDE) and DNA modified HMDE were used for the study of the interaction of 4‐nitrobiphenyl (4‐NBP) with double‐stranded DNA and for the detection of DNA damage caused by its interaction with 4‐NBP. Moreover, the interaction of DNA with intermediates of the 4‐NBP reduction and with 4‐aminobiphenyl, a metabolite of 4‐NBP, was investigated. The mechanism of the electrochemical reduction of 4‐NBP was investigated using CV, too. It was found that the reduction of 4‐NBP is controlled by both diffusion and adsorption. The rate constant of the reduction and the diffusion coefficient were calculated as well.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201600241