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Sensitive Electroanalytical Detection on GCE: the Case of Lipoic Acid and its Interaction with N-acetylcysteine and Glutathione

A simple and sensitive electrochemical method was developed for the determination of N‐Acetylcysteine (NAC) and Glutathione (GSH), on glassy carbon electrode. The results indicate that α‐lipoic acid (LipS2) is oxidized at the electrode, but is regenerated by thiols, suggesting a redox catalytic effe...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2016-11, Vol.28 (11), p.2818-2826
Main Authors: Santos, Ana Caroline F., Moura, Fabiana A., Tanaka, Auro A., Luz, Rita C. S., Damos, Flavio S., Kubota, Lauro T., Goulart, Marilia O. F.
Format: Article
Language:English
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Summary:A simple and sensitive electrochemical method was developed for the determination of N‐Acetylcysteine (NAC) and Glutathione (GSH), on glassy carbon electrode. The results indicate that α‐lipoic acid (LipS2) is oxidized at the electrode, but is regenerated by thiols, suggesting a redox catalytic effect. Under optimized conditions, the analytical curves for NAC and GSH were obtained in the range of 1 up to 10 µM and 1 up to 60 µM, respectively. The quantification can be performed in a single assay with high sensitivity, reproducibility and low detection limit of 0.27 and 0.67 µM for NAC and GSH, respectively. This method was successfully applied to the determination of NAC and GSH in artificial saliva and human urine samples, with excellent recovery results.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201600205