Skeletal muscle derived stem cells microintegrated into a biodegradable elastomer for reconstruction of the abdominal wall

Abstract A variety of techniques have been applied to generate tissue engineered constructs, where cells are combined with degradable scaffolds followed by a period of in vitro culture or direct implantation. In the current study, a cellularized scaffold was generated by concurrent deposition of ele...

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Bibliographic Details
Published in:Biomaterials 2017-01, Vol.113, p.31-41
Main Authors: Takanari, Keisuke, Hashizume, Ryotaro, Hong, Yi, Amoroso, Nicholas J, Yoshizumi, Tomo, Gharaibeh, Burhan, Yoshida, Osamu, Nonaka, Kazuhiro, Sato, Hideyoshi, Huard, Johnny, Wagner, William R
Format: Article
Language:English
Subjects:
Abdominal Wall - blood supply
Abdominal Wall - pathology
Abdominal Wall - physiology
Advanced Basic Science
Animals
Biocompatible Materials - chemistry
Biodegradability
Biodegradable
Cells, Cultured
Culture
Dentistry
Elastomer
Elastomers
Elastomers - chemistry
Electrospinning
Female
Gene expression
Muscle derived stem cells
Muscle, Skeletal - cytology
Polyurethane
Polyurethanes - chemistry
Rats
Rats, Sprague-Dawley
Rats, Transgenic
Regeneration
Scaffold
Scaffolds
Stem Cell Transplantation
Stem cells
Stem Cells - cytology
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Wound Healing
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Summary:Abstract A variety of techniques have been applied to generate tissue engineered constructs, where cells are combined with degradable scaffolds followed by a period of in vitro culture or direct implantation. In the current study, a cellularized scaffold was generated by concurrent deposition of electrospun biodegradable elastomer (poly(ester urethane)urea, PEUU) and electrosprayed culture medium + skeletal muscle-derived stem cells (MDSCs) or electrosprayed culture medium alone as a control. MDSCs were obtained from green fluorescent protein (GFP) transgenic rats. The created scaffolds were implanted into allogenic strain-matched rats to replace a full thickness abdominal wall defect. Both control and MDSC-integrated scaffolds showed extensive cellular infiltration at 4 and 8 wk. The number of blood vessels was higher, the area of residual scaffold was lower, number of multinucleated giant cells was lower and area of connective tissue was lower in MDSC-integrated scaffolds (p 
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2016.10.029