Blue-light Fourier-domain optical-coherence microscopy with linear k-sampling using second-harmonic generation

We demonstrate Fourier-domain optical-coherence microscopy (OCM) method that uses blue light for high-resolution microscopic imaging. Spectrally broad bandwidth is obtained by means of second-harmonic generation of Ti:sapphire laser light on the nonlinear crystal. Angular scanning of the crystal per...

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Bibliographic Details
Published in:Optics letters 2015-08, Vol.40 (15), p.3540-3543
Main Authors: Kolenderska, Sylwia M, Wilczyński, Grzegorz, Wojtkowski, Maciej
Format: Article
Language:English
Subjects:
Bandwidth
Crystals
Female
Fourier Analysis
Humans
Imaging
Lasers
Microscopy
Microscopy - methods
Optical Phenomena
Ovarian Neoplasms - pathology
Scanning
Spectra
Wavelengths
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Summary:We demonstrate Fourier-domain optical-coherence microscopy (OCM) method that uses blue light for high-resolution microscopic imaging. Spectrally broad bandwidth is obtained by means of second-harmonic generation of Ti:sapphire laser light on the nonlinear crystal. Angular scanning of the crystal performed by a resonant scanner results in second-harmonic generation for a broad range of frequencies producing blue light with central wavelength of 402 nm and bandwidth of 35 nm in one cycle. The axial resolution of the new setup is 3.5 μm in air, and the transverse resolution for Olympus 40× objective lens is 2.7 μm in X direction and 3.2 μm in Y direction. The developed technique enables registering spectral interferometric signal directly in k domain. Additionally, we present examples of imaging a biological specimen using the newly developed method.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.40.003540