3D printing of PLGA scaffolds for tissue engineering

• Published in: Journal of biomedical materials research. Part A 2017-01, Vol.105 (1), p.104-109
• Main Authors: Mironov, Anton V., Grigoryev, Aleksey M., Krotova, Larisa I., Skaletsky, Nikolaj N., Popov, Vladimir K., Sevastianov, Viktor I.
• Format: Article
• Language: English
• Subjects: 3D printing; Animals; Biocompatibility; Biomedical materials; bioresorbable polymeric scaffolds; Culture; Fibroblasts - cytology; Fibroblasts - metabolism; Islets of Langerhans - cytology; Islets of Langerhans - metabolism; Lactic Acid - chemistry; Materials Testing; Mathematical models; Mice; NIH 3T3 Cells; Polyglycolic Acid - chemistry; polylactic‐co‐glycolic acids; Printing, Three-Dimensional; Rabbits; Scaffolds; Surgical implants; Three dimensional printing; Tissue engineering; Tissue Scaffolds - chemistry
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35871

We proposed a novel method of generation of bioresorbable polymeric scaffolds with specified architectonics for tissue engineering using extrusion three‐dimensional (3D) printing with solutions of polylactoglycolide in tetraglycol with their subsequent solidifying in aqueous medium. On the basis of 3D computer models, we obtained the matrix structures with interconnected system of pores ranging in size from 0.5 to 500 µm. The results of in vitro studies using cultures of line NIH 3Т3 mouse fibroblasts, floating islet cultures of newborn rabbit pancreas, and mesenchymal stem cells of human adipose tissue demonstrated the absence of cytotoxicity and good adhesive properties of scaffolds in regard to the cell cultures chosen. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 104–109, 2017.