Use of the cytokinesis-block micronucleus assay to measure radiation-induced chromosome damage in lymphoblastoid cell lines

To establish the optimal experimental conditions for the use of the micronuclei (MN) test to determine the level of chromosomal damage induced by ionising radiation (IR) exposure in lymphoblastoid cell lines, a time-course study was performed comparing a normal and an ataxia telangiectasia (AT) cell...

Full description

Saved in:
Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2003-02, Vol.535 (1), p.1-13
Main Authors: Gutiérrez-Enrı&#x0301, quez, Sara, Hall, Janet
Format: Article
Language:English
Subjects:
Ataxia telangiectasia gene ( ATM)
Biological and medical sciences
Cytochalasin-B
Fundamental and applied biological sciences. Psychology
Lymphoblastoid cell lines
Medical sciences
Miscellaneous
Molecular and cellular biology
Molecular genetics
Mutagenesis. Repair
Radiation-induced micronuclei
Toxicology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To establish the optimal experimental conditions for the use of the micronuclei (MN) test to determine the level of chromosomal damage induced by ionising radiation (IR) exposure in lymphoblastoid cell lines, a time-course study was performed comparing a normal and an ataxia telangiectasia (AT) cell line, the latter being characterised by an extreme radiation sensitivity. Several parameters were analysed: the use of cytochalasin-B (Cyt-B) to quantify MN, the optimum fixation time to measure radiation-induced MN, the most appropriate treatment dose of IR to distinguish between the normal and the radiosensitive cells and the cell-cycle distribution after irradiation. The results obtained showed that the spontaneous as well as the radiation-induced levels of MN were significantly higher in the AT cell compared to the normal cells ( P
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(02)00280-2