Influence of partition parameters on a recombinant antigen of Schistosoma mansoni expressed on E. coli using poly(ethylene glycol)-hydroxypropyl starch aqueous two-phase system

This work describes the partition of a Schistosoma mansoni tegumental antigen produced by a recombinant Escherichia coli strain using an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and purified hydroxypropyl-starch (Reppal PES 100). The effects of the polymer molecular weig...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2002-10, Vol.18 (7), p.645-648
Main Authors: CHAVES, A. C, SILVEIRA, E, BEZERRA, R. P, MOREIRA, K. A, LUCENA-SILVA, N. L. C, ABATH, F. G. C, PORTO, A. L. F, CABRAL, J. M. S, LIMA-FILHO, J. L
Format: Article
Language:English
Subjects:
Antigens
Bacteria
Biological and medical sciences
E coli
Fundamental and applied biological sciences. Psychology
Microbiology
Molecular weight
Polyethylene glycol
Polymers
Starch
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Summary:This work describes the partition of a Schistosoma mansoni tegumental antigen produced by a recombinant Escherichia coli strain using an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) and purified hydroxypropyl-starch (Reppal PES 100). The effects of the polymer molecular weight, tie line length and pH on antigen partitioning were investigated. The detection of the antigen in both phases was determined by ELISA. The system composed of PEG 8000 (5.1% w/w) and Reppal PES 100 (13.0% w/w) led to a yield of 92% and a purification factor of 12 concerning the antigen in the PEG-rich phase. It was observed that antigen partition in ATPSs was strongly affected by the pH and tie line length. In addition, it was possible in a single step, to remove the cell debris, which precipitated at the interface of the system.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1023/A:1016887617041