Flow injection amperometric detection of OP nerve agents based on an organophosphorus–hydrolase biosensor detector

A flow-injection system with an organophosphorus–hydrolase (OPH)-biosensor detector has been developed and characterized for the rapid detection of organophosphorus (OP) nerve agents. The enzyme was immobilized onto a thin-film gold detector through a cystamine–glutaraldehyde coupling. Factors influ...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2003-03, Vol.18 (2), p.255-260
Main Authors: Wang, Joseph, Krause, Robin, Block, Kirstin, Musameh, Mustafa, Mulchandani, Ashok, Schöning, Michael J
Format: Article
Language:English
Subjects:
Aryldialkylphosphatase
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensor detector
Biosensors
Biotechnology
Cholinesterase Inhibitors - analysis
Coated Materials, Biocompatible - chemical synthesis
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Enzymes, Immobilized
Equipment Design
Esterases
Flow injection
Flow Injection Analysis - instrumentation
Flow Injection Analysis - methods
Fundamental and applied biological sciences. Psychology
Insecticides - analysis
Methods. Procedures. Technologies
Organophosphorus
Organophosphorus Compounds - analysis
Paraoxon - analysis
Reproducibility of Results
Sensitivity and Specificity
Various methods and equipments
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Summary:A flow-injection system with an organophosphorus–hydrolase (OPH)-biosensor detector has been developed and characterized for the rapid detection of organophosphorus (OP) nerve agents. The enzyme was immobilized onto a thin-film gold detector through a cystamine–glutaraldehyde coupling. Factors influencing the performance were optimized. The resulting flow system offered a fast, sensitive, selective, and stable response. The peak current increased linearly with the concentration of paraoxon and methyl parathion over the 1–10 μM range (sensitivity, 2.29 and 1.04 nA/μM, respectively). The OPH-biosensor flow injection systems offered low detection limits (e.g. 0.1 μM paraoxon), along with a good precision (R.S.D. of 3.6% for 20 successive injections of a 1.0 μM paraoxon solution). The OPH-biosensor flow detector offers great promise for rapid field screening of OP pesticides and nerve agents.
ISSN:0956-5663
1873-4235
DOI:10.1016/S0956-5663(02)00178-1