High-Throughput Quantitation of Proline Betaine in Foods and Suitability as a Valid Biomarker for Citrus Consumption

Proline betaine has been proposed as a candidate dietary biomarker for citrus intake. To validate its suitability as a dietary biomarker and to gain insight into the range of this per-methylated amino acid in foods and beverages, a quick and accurate stable isotope dilution assay was developed for q...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2017-03, Vol.65 (8), p.1613-1619
Main Authors: Lang, Roman, Lang, Tatjana, Bader, Matthias, Beusch, Anja, Schlagbauer, Verena, Hofmann, Thomas
Format: Article
Language:English
Subjects:
Adult
Betaine - metabolism
Betaine - urine
Beverages - analysis
Biomarkers - metabolism
Biomarkers - urine
Citrus - metabolism
Female
Fruit - metabolism
High-Throughput Screening Assays
Humans
Male
Proline - metabolism
Proline - urine
Tandem Mass Spectrometry
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Proline betaine has been proposed as a candidate dietary biomarker for citrus intake. To validate its suitability as a dietary biomarker and to gain insight into the range of this per-methylated amino acid in foods and beverages, a quick and accurate stable isotope dilution assay was developed for quantitative high-throughput HILIC-MS/MS screening of proline betaine in foods and urine after solvent-mediated matrix precipitation. Quantitative analysis of a variety of foods confirmed substantial amounts of proline betaine in citrus juices (140–1100 mg/L) and revealed high abundance in tubers of the vegetable Stachys affinis, also known as Chinese artichocke (∼700 mg/kg). Seafood including clams, shrimp, and lobster contained limited amounts (1–95 mg/kg), whereas only traces were detected in fish, cuttlefish, fresh meat, dairy products, fresh vegetable (
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.6b05824