Gene analysis of two cases with CisAB/B blood subgroup

Abstract Objective To identify the precise ABO blood type subgroups with genotyping. Method We screened ABO blood type from voluntary blood donors in our blood collection center. Samples were first examined with a routine serological method. Samples with ambiguous results were further examined with...

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Bibliographic Details
Published in:Transfusion and apheresis science 2017-04, Vol.56 (2), p.223-225
Main Authors: Jiao, Li-xin, Yu, Jiang-hong, Yu, Xiao-li, Chen, Lin, Han, Yu, Yang, Fan
Format: Article
Language:English
Subjects:
ABO blood subgroup
ABO Blood-Group System - genetics
Adult
Blood Donors
CisAB blood type
Genotype
Genotyping Techniques
Health technology assessment
Hematology, Oncology and Palliative Medicine
Humans
Male
Middle Aged
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Summary:Abstract Objective To identify the precise ABO blood type subgroups with genotyping. Method We screened ABO blood type from voluntary blood donors in our blood collection center. Samples were first examined with a routine serological method. Samples with ambiguous results were further examined with genotyping to identify ABO subgroups. Results Two samples identified as Aweak B by serology were considered to be A/B and B/B by PCR-SSP. However, the gene sequencing results revealed the precise subgroup to be CisAB01 /B101 and CisAB05 /B101 , respectively. Conclusion It may be difficult to identify non-typical AB patients with a routine serological method. Genotyping is a more precise method to identify blood subgroups.
ISSN:1473-0502
DOI:10.1016/j.transci.2016.12.002