Acclimation of shade-tolerant and light-resistant Tradescantia species to growth light: chlorophyll a fluorescence, electron transport, and xanthophyll content

• Published in: Photosynthesis research 2017-09, Vol.133 (1-3), p.87-102
• Main Authors: Mishanin, Vladimir I., Trubitsin, Boris V., Patsaeva, Svetlana V., Ptushenko, Vasily V., Solovchenko, Alexei E., Tikhonov, Alexander N.
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization - physiology; Acclimatization - radiation effects; Analysis; Anatomy; Biochemistry; Biomedical and Life Sciences; Carotenoids; Chlorophyll; Chlorophyll - metabolism; Chloroplasts; Darkness; Electron transport; Electron Transport - radiation effects; Flowers & plants; Fluorescence; Fluorometry; Growth; Kinetics; Life Sciences; Light; Original Article; Oxidation; Oxidation-Reduction; Photons; Photosynthesis; Photosynthetic apparatus; Plant Genetics and Genomics; Plant Leaves - metabolism; Plant Leaves - radiation effects; Plant Physiology; Plant Sciences; Shade; Spectrometry, Fluorescence; Time Factors; Tradescantia - growth & development; Tradescantia - physiology; Tradescantia - radiation effects; Violaxanthin; Xanthophylls; Xanthophylls - metabolism; Zeaxanthin
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-017-0339-1

In this study, we have compared the photosynthetic characteristics of two contrasting species of Tradescantia plants, T. fluminensis (shade-tolerant species), and T. sillamontana (light-resistant species), grown under the low light (LL, 50–125 µmol photons m −2  s −1 ) or high light (HL, 875–1000 µmol photons m −2  s −1 ) conditions during their entire growth period. For monitoring the functional state of photosynthetic apparatus (PSA), we measured chlorophyll (Chl) a emission fluorescence spectra and kinetics of light-induced changes in the heights of fluorescence peaks at 685 and 740 nm ( F 685 and F 740 ). We also compared the light-induced oxidation of P 700 and assayed the composition of carotenoids in Tradescantia leaves grown under the LL and HL conditions. The analyses of slow induction of Chl a fluorescence (SIF) uncovered different traits in the LL- and HL-grown plants of ecologically contrasting Tradescantia species, which may have potential ecophysiological significance with respect to their tolerance to HL stress. The fluorometry and EPR studies of induction events in chloroplasts in situ demonstrated that acclimation of both Tradescantia species to HL conditions promoted faster responses of their PSA as compared to LL-grown plants. Acclimation of both species to HL also caused marked changes in the leaf anatomy and carotenoid composition (an increase in Violaxanthin + Antheraxantin + Zeaxanthin and Lutein pools), suggesting enhanced photoprotective capacity of the carotenoids in the plants grown in nature under high irradiance. Collectively, the results of the present work suggest that the mechanisms of long-term PSA photoprotection in Tradescantia are based predominantly on the light-induced remodeling of pigment-protein complexes in chloroplasts.