Production of reactive oxygen species by toxin T-514 of genus Karwinskia in vitro

In the present study we have analyzed the production of reactive oxygen species by toxin T-514 of the genus Karwinskia in vitro (primary liver cell cultures and microsomes), as well as their possible role in its cytotoxicity. The role of catalase and superoxide dismutase (SOD) as defense mechanisms...

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Bibliographic Details
Published in:Toxicology in vitro 2003-02, Vol.17 (1), p.19-25
Main Authors: Garza-Ocañas, L, Zanatta-Calderón, M.T, Acosta, D, Torres-Alanı́s, O, Piñeyro-López, A
Format: Article
Language:English
Subjects:
Animals
Anthracenes - adverse effects
Biological and medical sciences
Catalase - pharmacology
Cytotoxicity in vitro
Cytotoxins
Hepatocytes
Karwinskia - chemistry
Liver - cytology
Male
Medical sciences
Microsomes, Liver
Oxidative Stress
Plant poisons toxicology
Radical scavengers
Rats
Rats, Sprague-Dawley
Rats, Wistar
Reactive Oxygen Species
Superoxide Dismutase - pharmacology
Toxicology
Toxin T-514
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Summary:In the present study we have analyzed the production of reactive oxygen species by toxin T-514 of the genus Karwinskia in vitro (primary liver cell cultures and microsomes), as well as their possible role in its cytotoxicity. The role of catalase and superoxide dismutase (SOD) as defense mechanisms against oxidative stress was also studied. Freshly isolated hepatocytes or microsomes were exposed to T-514 in the presence or absence of catalase and SOD. Cytotoxicity was determined by methylthiazoltetrazolium (MTT) reduction. Oxidative stress was evaluated by the dichlorofluorescein diacetate (DCFDA) fluorescent probe and the reduction of ferricytochrome c. Exposure of hepatocytes to toxin T-514 for 2-, 4-, 6- and 24-h periods resulted in a time- and concentration-dependent increase in the suppression of mitochondrial metabolic activity. T-514 induced the production of reactive oxygen species in both hepatocytes and microsomes. Catalase and superoxide dismutase had a protective effect against the cytotoxicity of T-514 in hepatocytes and also inhibited the production of oxygen reactive species in microsomes. The results indicate that oxidative stress mediated by reactive intermediates may be a mechanism by which T-514 induces its cytotoxic effect.
ISSN:0887-2333
1879-3177
DOI:10.1016/S0887-2333(02)00126-1