DNA fingerprinting and virulence analysis of Xanthomonas oryzae pv. oryzae isolates from Punjab, northern India

• Published in: Euphytica 2003-01, Vol.130 (1), p.107-115
• Main Authors: SUKHWINDER-SINGH, SODHI, M, VIKAL, Y, GEORGE, M. L. C, BALM, G. S, MANGAT, G. S, GANG, M, SIDHU, J. S, DHALIWAL, H. S
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Blight; Deoxyribonucleic acid; DNA; DNA fingerprinting; Fundamental and applied biological sciences. Psychology; Genes; Genetic fingerprinting; Genetics; Genetics and breeding of economic plants; Haplotypes; Mathematics; Pathogens; Pest resistance; Plant pathogens; Varietal selection. Specialized plant breeding, plant breeding aims; Virulence; Xanthomonas oryzae
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1022329024651

DNAs of 693 isolates of bacterial blight pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo), were characterized using PCR-based primers pJEL1 and pJEL2. The pathogen populations were grouped into 97 haplotypes based on DNA-banding patterns. An un-weighted pair-group method using arithmetic averages (UPGMA) indicated a high level of diversity in the pathogen isolates (51 lineages of Xoo at a 70% similarity level). Among these, lineages 5, 7, 27, and 29 are widely distributed and others are localized in the northern region of India. The isolates represent lineage-27, were prevalent in the entire disease-prone area in the region except at Ferozepur. Pathotyping data of the representative isolates of each lineage also indicate 17 different reaction patterns on a set of isogenic lines. Resistance genes xa8 and Xa21 were the most effective followed by xa5, and Xa7 against Xoo isolates prevalent in northern India. Different genes in combinations (xa5+xa13, xa5+Xa21, xa13+Xa21, and xa5+xa13+Xa21) in IR24 genetic background provided better protection against all the pathogen isolates tested in this study than did the component genes.