ITS and β-tubulin markers help delineate Phaeoacremonium species, and the occurrence of P. parasiticum in grapevine disease in Argentina

Restrictions patterns of the ITS rDNA and a partial fragment of the β-tubulin gene were shown to help identify Phaeoacremonium species associated with diseased grapevines. PCR-RFLP markers revealed a high frequency of P. parasiticum, together with P. aleophilum, in fungi isolated from stems and trun...

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Bibliographic Details
Published in:Mycological research 2002-10, Vol.106 (10), p.1143-1150
Main Authors: DUPONT, Joëlle, MAGNIN, Sandrine, CÉSARI, Cecilia, GATICA, Marta
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
fungi
genes
internal transcribed spacers
Phytopathology. Animal pests. Plant and forest protection
polymerase chain reaction
restriction fragment length polymorphism
ribosomal DNA
stems
Systematics. Morphology. Development cycle. Physiology
tubulin
vines
vineyards
Vitis
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Summary:Restrictions patterns of the ITS rDNA and a partial fragment of the β-tubulin gene were shown to help identify Phaeoacremonium species associated with diseased grapevines. PCR-RFLP markers revealed a high frequency of P. parasiticum, together with P. aleophilum, in fungi isolated from stems and trunks of mature diseased vines showing ‘hoja de malvón’ disease, and from young declining plants in Argentina. This distribution is different from that in vineyards in other parts of the world, where P. aleophilum is dominant. Indeed, this is the first report associating P. parasiticum with diseased vines.
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756202006639