PRODUCTION OF ADVENTITIOUS SHOOTS AND PLANTLETS FROM THE HYPOCOTYL EXPLANTS OF SESBANIA ROSTRATA (BREMEK & OBREM.)

• Published in: In vitro cellular & developmental biology. Plant 2002-09, Vol.38 (5), p.430-434
• Main Authors: JHA, AJAY KUMAR, PRAKASH, SURYA, JAIN, NEERU, NANDA, KANAN, GUPTA, S. C.
• Format: Article
• Language: English
• Subjects: Adventitious shoots; Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Callus; Developmental biology; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Hypocotyls; In vitro culture; Inoculation; Micropropagation; Plant cells; Plant physiology and development; Plantlets; Plants; Seedlings; Tissue cultures, protoplasts
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1079/ivp2002313

Adventitious shoots were induced from the hypocotyl explants derived from 12—15-d-old seedlings of Sesbania rostrata on Nitsch's medium (Nitsch, 1969) supplemented with 1 mg l—1 (4.4 μM) of N6-benzyladenine (BA). A maximum of 5.9 ± 3.4 shoots per explant in 100% of cultures were obtained. The BA treatment for different time durations (1, 3, 5, 7, 10, 17, 21, or 30 d) exhibited significant variation in the caulogenic potential of the explants. BA treatment for 10—17 d proved optimum for the response. Although at all concentrations of kinetin the explants developed multiple shoots, they were malformed. Sucrose at 3% exhibited the development of the maximum of 3.5 ± 0.9 shoots per explant with an average shoot length of 4.7 ± 3.9 cm. Among the different carbon sources, i.e., fructose, galactose, maltose, mannose, and sucrose at 3% (w/v), sucrose supported the best caulogenic response. The role of various other factors (viz. size, orientation of explant, and seedling age) on the caulogenic response of the hypocotyl explants of S. rostrata were also studied. The shoot development in all cases was accompanied by the development of moderate to profuse callus at the basal cut end of the explant. The in vitro-regenerated shoots produced roots when transferred to half-strength MS medium (Murashige and Skoog, 1962) supplemented with 3% sucrose and 1 mg l—1 (4.9 μM) indole-3-butyric acid (IBA). The developed plantlets were transferred to the field after an initial acclimatization period of 3—4 mo. Such transferred plants produced flowers and fruits in the field and exhibited the development of prominent and organized stem nodules.