The Cu,Zn superoxide dismutase in neuroblastoma SK-N-BE cells is exported by a microvesicles dependent pathway

The antioxidant enzyme Cu,Zn superoxide dismutase has so far been considered costitutively expressed and exclusively localized into cytosol. In this paper we investigated Cu,Zn superoxide dismutase export in neuroblastoma SK-N-BE cells by flow cytometry analysis, confocal immunofluorescence analysis...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 2003-01, Vol.110 (1), p.45-51
Main Authors: Mondola, Paolo, Ruggiero, Giuseppina, Serù, Rosalba, Damiano, Simona, Grimaldi, Serena, Garbi, Corrado, Monda, Marcellino, Greco, Dario, Santillo, Mariarosaria
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Western
Brefeldin A - pharmacology
Cell physiology
Cu,Zn superoxide dismutase
Cytoplasmic Vesicles - physiology
Cytoskeleton - metabolism
Deoxyglucose - pharmacology
Enzyme Inhibitors - pharmacology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Humans
Membrane and intracellular transports
Methylamines - pharmacology
Microvesicles
Molecular and cellular biology
Neuroblastoma
Protein export
Protein Synthesis Inhibitors - pharmacology
Protein Transport - drug effects
Protein Transport - physiology
Sodium Azide - pharmacology
Superoxide Dismutase - metabolism
Tumor Cells, Cultured
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Summary:The antioxidant enzyme Cu,Zn superoxide dismutase has so far been considered costitutively expressed and exclusively localized into cytosol. In this paper we investigated Cu,Zn superoxide dismutase export in neuroblastoma SK-N-BE cells by flow cytometry analysis, confocal immunofluorescence analysis and enzyme-linked immunosorbed assay. Immunofluorescence analysis shows that the enzyme is exported by microvesicular granules; moreover the treatment of cells with brefeldin A and with 2-deoxy- d-glucose and sodium azide strongly decreases the amount of CuZn superoxide dismutase detected in the medium. Therefore the involvement of ATP-dependent mechanisms, likely including BFA-sensitive intracytoplasmic vesicles in Cu,Zn SOD export from SK-N-BE cells, has to be hypothesized. Microvesicular-mediated Cu,Zn SOD export in neurons could represent a relevant phenomenon able to influence cell excitability that is affected by reactive oxygen species.
ISSN:0169-328X
1872-6941
DOI:10.1016/S0169-328X(02)00583-1