Proliferation of canine bone marrow derived mesenchymal stem cells on different nanomaterial based thin film scaffolds

• Published in: Tissue & cell 2017-04, Vol.49 (2), p.270-274
• Main Authors: Das, Kinsuk, Mili, Bhabesh, A.P., Madhusoodan, Saxena, Abhishek Chandra, Kumar, Ajay, Singh, Praveen, Verma, Med Ram, Sarkar, Mihir, Bag, Sadhan
• Format: Article
• Language: English
• Subjects: Advanced Basic Science; Animals; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Canine MSCs; Caproates - administration & dosage; Caproates - chemistry; Carbon nanotubes; Cell culture; Cell Differentiation - drug effects; Cell growth; Cell number; Cell proliferation; Cell Proliferation - drug effects; Chitosan; Chitosan - administration & dosage; Chitosan - chemistry; CNT; Dogs; Extracellular matrix; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; Lactones - administration & dosage; Lactones - chemistry; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchyme; Nanomaterials; Nanostructures - administration & dosage; Nanostructures - chemistry; Nanotechnology; Nanotubes; Nanotubes, Carbon - chemistry; Osteogenesis - drug effects; PCL; Proliferation; Scaffolds; Stem cells; Substrates; Thin films; Tissue Engineering; Tissue Scaffolds
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2017.02.002

Highlights • Mesenchymal stem cells (MSCs) were isolated from canine bone marrow, characterized and expanded in vitro. • Thin film scaffolds of carbon nanotubes (CNTs) and chitosan nanoparticles were prepared and characterized. • Canine MSCs proliferation study was done onto these scaffolds and aligned nanofibers matrixes of electrospun poly ε-caprolactone (PCL). • Nanomaterial based scaffolds reduced the cell proliferation rate but no decline of cell number was noticed during the study period. • Canine MSCs adapted and proliferated better on carbon nanotube scaffolds than the two other polymeric substrates.