The cryoprotective effect of iodixanol in buffalo semen cryopreservation

•2.5% OptiPrepTM in extender improved sperm motility, plasma membrane integrity and 7distance travelled by sperm in cervical mucus.•Total antioxidant capacity of 2.5 and 5% OptiPrep™ treated sperm were found elevated.•Lipid peroxidation found lowest in OptiPrep™ treated sperm.•In incubation test, 2....

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Bibliographic Details
Published in:Animal reproduction science 2017-04, Vol.179, p.20-26
Main Authors: Swami, Dheer Singh, Kumar, Pradeep, Malik, R.K., Saini, Monika, Kumar, Dharmendra, Jan, M.H.
Format: Article
Language:English
Subjects:
Animals
Buffalo
Buffaloes
CASA
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Iodixanol
Male
OptiPrepTM
Oxidative stress
Semen
Semen - drug effects
Semen Preservation - veterinary
Triiodobenzoic Acids - pharmacology
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Summary:•2.5% OptiPrepTM in extender improved sperm motility, plasma membrane integrity and 7distance travelled by sperm in cervical mucus.•Total antioxidant capacity of 2.5 and 5% OptiPrep™ treated sperm were found elevated.•Lipid peroxidation found lowest in OptiPrep™ treated sperm.•In incubation test, 2.5% OptiPrep™ preserved better sperm motility.•In conclusion, 2.5% OptiPrepTM supplementation resulting overall improvement in the post-thaw semen quality. This is the first report to examine the effect of iodixanol (OptiPrepTM) on cryosurvival of buffalo spermatozoa. A total of thirty ejaculates (five ejaculates from each bull) from six buffalo bulls were used for this experiment. Each ejaculate was divided into four aliquots and diluted in freezing extender supplemented with different concentrations of OptiPrepTM (0, 1.25, 2.5 and 5%) and then cryopreserved. The semen quality variables were evaluated before and after freezing of the semen. There were no effects of OptiPrepTM (P>0.05) on sperm kinetics, motility, abnormality and membrane integrity of fresh extended spermatozoa. However, after freeze-thaw, sperm motility, plasma membrane integrity and distance travelled in cervical mucus of 2.5% OptiPrepTM treated samples showed significantly higher (P0.05) were seen in sperm abnormality and acrosomal integrity of treated and control frozen-thawed samples. The total antioxidant capacity of 2.5 and 5% OptiPrepTM treated frozen-thawed sperm were found to be higher (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2017.01.012