MALDI-TOF mass spectrometry: Evaluation of the preanalytical phase for identification of molds

In order to optimize the identification of molds with MALDI-TOF MS, three protein extraction-methodologies were evaluated against 44 isolates: water extraction (WE), zirconium extraction (ZE) and the provider's recommended method (PRM). Two data bases were compared, Bruker (BK) and Bruker+Natio...

Full description

Saved in:
Bibliographic Details
Published in:Revista argentina de microbiología 2017-01, Vol.49 (1), p.7-14
Main Authors: Maldonado, Ivana, García Ramírez, Dolores, Striebeck, Pablo, Lafage, Marcelo, Fernández Canigia, Liliana
Format: Article
Language:Spanish
Subjects:
Databases, Factual
Fungi - isolation & purification
Pre-Analytical Phase
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to optimize the identification of molds with MALDI-TOF MS, three protein extraction-methodologies were evaluated against 44 isolates: water extraction (WE), zirconium extraction (ZE) and the provider's recommended method (PRM). Two data bases were compared, Bruker (BK) and Bruker+National Institutes of Health. Considering both databases, results were respectively as follows: correct identification (CI) at gender level, 10 and 16 by WE; 27 and 32 by ZE and 18 and 23 by PRM; CI at species level, 5 and 7 by WE; 17 and 20 by ZE and 11 and 14 by PRM; non-reliable identification, 18 and 12 by WE; 9 and 4 by ZE and by PRM. No peaks were observed in 16 by WE, 8 by ZE and 17 by PRM. ZE showed the best perfomance (p
ISSN:0325-7541
DOI:10.1016/j.ram.2016.10.001