Loading…
In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Accelerated Terminal Differentiation in Fetal Mouse Skin
2,3,7,8 Tetrachlorodibenzo-p-dioxin (TCDD), a ubiquitous environmental toxin, has been shown to cause a human skin pathology called chloracne. The majority of laboratory mouse strains, with the exception of mice bearing a mutation in thehairless gene, fail to display overt signs of chloracne upon ex...
Saved in:
Published in: | Toxicological sciences 2002-08, Vol.68 (2), p.465-472 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c499t-23562125cc064f3a8bdbed799743881ce49f763461e0516e8ebceae4e19ba4af3 |
---|---|
cites | |
container_end_page | 472 |
container_issue | 2 |
container_start_page | 465 |
container_title | Toxicological sciences |
container_volume | 68 |
creator | Loertscher, Jennifer A. Lin, Tien-Min Peterson, Richard E. Allen-Hoffmann, B. Lynn |
description | 2,3,7,8 Tetrachlorodibenzo-p-dioxin (TCDD), a ubiquitous environmental toxin, has been shown to cause a human skin pathology called chloracne. The majority of laboratory mouse strains, with the exception of mice bearing a mutation in thehairless gene, fail to display overt signs of chloracne upon exposure to TCDD. As a result, only minimal data exist on the effects of TCDD in adult haired mice and no data exist on the effects of TCDD in developing mouse skin. Here we report that TCDD affects the temporal expression of protein markers of keratinocyte terminal differentiation during murine skin morphogenesis. Immunohistochemical analysis of E16 mice reveals accelerated expression of the intermediate filament-associated protein filaggrin in response to TCDD. At a later developmental time and after birth, expression of filaggrin and loricrin is indistinguishable between treatment and control groups. At E16 expression of keratins 5, 6, and 10 are unaltered in TCDD-exposed individuals and TUNEL analysis shows no apoptotic cells in the basal and spinous layers of either treatment or control groups. At E16, immunohistochemical analysis of AhR-null mouse skin reveals accelerated filaggrin expression in both vehicle and TCDD exposed animals. We therefore hypothesize that AhR acts as a modulator of late stage keratinocyte terminal differentiation. |
doi_str_mv | 10.1093/toxsci/68.2.465 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_18679855</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18679855</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-23562125cc064f3a8bdbed799743881ce49f763461e0516e8ebceae4e19ba4af3</originalsourceid><addsrcrecordid>eNpFkM1vEzEQxS0EoqVw5oZ8gVM28ceu1z5WoSWVgqAilVAvltc7K0w3drAdaeGvx1VW7WlGM783evMQek_JkhLFVzlMybqVkEu2rEXzAp2XsaiIYurl3AsiyRl6k9JvQigVRL1GZ5TRhoqan6PpxuO7DDHgq-kQ0jECzgGzBV-0C1ntIEdjf40hht514P-F6lD1LkzO47U5Jkj40loYIZoMPd5B3DtvRvzZDQNE8NmZ7ILHBb-GXBZfQxHhHw_Ov0WvBjMmeDfXC3R3fbVbb6rtty8368ttZWulcsV4IxhljbVE1AM3sus76Ful2ppLSS3UamgFrwUFUl4CCZ0FAzVQ1ZnaDPwCfTrdPcTw5wgp671LxfJoPBQzmkrRKtk0BVydQBtDShEGfYhub-JfTYl-DFufwtZCaqZL2EXxYT597PbQP_NzugX4OAMmWTMO0Xjr0jNXPiAtZ4WrTpxLGaanvYkPWrS8bfTm573e3ZPvW357qzf8P2QWmQk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18679855</pqid></control><display><type>article</type><title>In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Accelerated Terminal Differentiation in Fetal Mouse Skin</title><source>Oxford Journals Online</source><source>Free Full-Text Journals in Chemistry</source><creator>Loertscher, Jennifer A. ; Lin, Tien-Min ; Peterson, Richard E. ; Allen-Hoffmann, B. Lynn</creator><creatorcontrib>Loertscher, Jennifer A. ; Lin, Tien-Min ; Peterson, Richard E. ; Allen-Hoffmann, B. Lynn</creatorcontrib><description>2,3,7,8 Tetrachlorodibenzo-p-dioxin (TCDD), a ubiquitous environmental toxin, has been shown to cause a human skin pathology called chloracne. The majority of laboratory mouse strains, with the exception of mice bearing a mutation in thehairless gene, fail to display overt signs of chloracne upon exposure to TCDD. As a result, only minimal data exist on the effects of TCDD in adult haired mice and no data exist on the effects of TCDD in developing mouse skin. Here we report that TCDD affects the temporal expression of protein markers of keratinocyte terminal differentiation during murine skin morphogenesis. Immunohistochemical analysis of E16 mice reveals accelerated expression of the intermediate filament-associated protein filaggrin in response to TCDD. At a later developmental time and after birth, expression of filaggrin and loricrin is indistinguishable between treatment and control groups. At E16 expression of keratins 5, 6, and 10 are unaltered in TCDD-exposed individuals and TUNEL analysis shows no apoptotic cells in the basal and spinous layers of either treatment or control groups. At E16, immunohistochemical analysis of AhR-null mouse skin reveals accelerated filaggrin expression in both vehicle and TCDD exposed animals. We therefore hypothesize that AhR acts as a modulator of late stage keratinocyte terminal differentiation.</description><identifier>ISSN: 1096-6080</identifier><identifier>ISSN: 1096-0929</identifier><identifier>EISSN: 1096-0929</identifier><identifier>DOI: 10.1093/toxsci/68.2.465</identifier><identifier>PMID: 12151643</identifier><identifier>CODEN: TOSCF2</identifier><language>eng</language><publisher>Cary, NC: Oxford University Press</publisher><subject>AhR ; Animals ; Animals, Newborn ; Biological and medical sciences ; Biomarkers ; Embryology: invertebrates and vertebrates. Teratology ; Environmental Pollutants - toxicity ; Female ; Fetus - drug effects ; Fetus - pathology ; filaggrin ; Fundamental and applied biological sciences. Psychology ; Intermediate Filament Proteins - metabolism ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Male ; Maternal Exposure - adverse effects ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Morphogenesis - drug effects ; Morphogenesis - physiology ; mouse ; Polychlorinated Dibenzodioxins - toxicity ; Pregnancy ; Receptors, Aryl Hydrocarbon - genetics ; skin ; Skin - drug effects ; Skin - embryology ; Skin - growth & development ; TCDD ; Teratology. Teratogens</subject><ispartof>Toxicological sciences, 2002-08, Vol.68 (2), p.465-472</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-23562125cc064f3a8bdbed799743881ce49f763461e0516e8ebceae4e19ba4af3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13880732$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12151643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Loertscher, Jennifer A.</creatorcontrib><creatorcontrib>Lin, Tien-Min</creatorcontrib><creatorcontrib>Peterson, Richard E.</creatorcontrib><creatorcontrib>Allen-Hoffmann, B. Lynn</creatorcontrib><title>In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Accelerated Terminal Differentiation in Fetal Mouse Skin</title><title>Toxicological sciences</title><addtitle>Toxicol. Sci</addtitle><description>2,3,7,8 Tetrachlorodibenzo-p-dioxin (TCDD), a ubiquitous environmental toxin, has been shown to cause a human skin pathology called chloracne. The majority of laboratory mouse strains, with the exception of mice bearing a mutation in thehairless gene, fail to display overt signs of chloracne upon exposure to TCDD. As a result, only minimal data exist on the effects of TCDD in adult haired mice and no data exist on the effects of TCDD in developing mouse skin. Here we report that TCDD affects the temporal expression of protein markers of keratinocyte terminal differentiation during murine skin morphogenesis. Immunohistochemical analysis of E16 mice reveals accelerated expression of the intermediate filament-associated protein filaggrin in response to TCDD. At a later developmental time and after birth, expression of filaggrin and loricrin is indistinguishable between treatment and control groups. At E16 expression of keratins 5, 6, and 10 are unaltered in TCDD-exposed individuals and TUNEL analysis shows no apoptotic cells in the basal and spinous layers of either treatment or control groups. At E16, immunohistochemical analysis of AhR-null mouse skin reveals accelerated filaggrin expression in both vehicle and TCDD exposed animals. We therefore hypothesize that AhR acts as a modulator of late stage keratinocyte terminal differentiation.</description><subject>AhR</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Environmental Pollutants - toxicity</subject><subject>Female</subject><subject>Fetus - drug effects</subject><subject>Fetus - pathology</subject><subject>filaggrin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Intermediate Filament Proteins - metabolism</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Male</subject><subject>Maternal Exposure - adverse effects</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Morphogenesis - drug effects</subject><subject>Morphogenesis - physiology</subject><subject>mouse</subject><subject>Polychlorinated Dibenzodioxins - toxicity</subject><subject>Pregnancy</subject><subject>Receptors, Aryl Hydrocarbon - genetics</subject><subject>skin</subject><subject>Skin - drug effects</subject><subject>Skin - embryology</subject><subject>Skin - growth & development</subject><subject>TCDD</subject><subject>Teratology. Teratogens</subject><issn>1096-6080</issn><issn>1096-0929</issn><issn>1096-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNpFkM1vEzEQxS0EoqVw5oZ8gVM28ceu1z5WoSWVgqAilVAvltc7K0w3drAdaeGvx1VW7WlGM783evMQek_JkhLFVzlMybqVkEu2rEXzAp2XsaiIYurl3AsiyRl6k9JvQigVRL1GZ5TRhoqan6PpxuO7DDHgq-kQ0jECzgGzBV-0C1ntIEdjf40hht514P-F6lD1LkzO47U5Jkj40loYIZoMPd5B3DtvRvzZDQNE8NmZ7ILHBb-GXBZfQxHhHw_Ov0WvBjMmeDfXC3R3fbVbb6rtty8368ttZWulcsV4IxhljbVE1AM3sus76Ful2ppLSS3UamgFrwUFUl4CCZ0FAzVQ1ZnaDPwCfTrdPcTw5wgp671LxfJoPBQzmkrRKtk0BVydQBtDShEGfYhub-JfTYl-DFufwtZCaqZL2EXxYT597PbQP_NzugX4OAMmWTMO0Xjr0jNXPiAtZ4WrTpxLGaanvYkPWrS8bfTm573e3ZPvW357qzf8P2QWmQk</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Loertscher, Jennifer A.</creator><creator>Lin, Tien-Min</creator><creator>Peterson, Richard E.</creator><creator>Allen-Hoffmann, B. Lynn</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20020801</creationdate><title>In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Accelerated Terminal Differentiation in Fetal Mouse Skin</title><author>Loertscher, Jennifer A. ; Lin, Tien-Min ; Peterson, Richard E. ; Allen-Hoffmann, B. Lynn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-23562125cc064f3a8bdbed799743881ce49f763461e0516e8ebceae4e19ba4af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>AhR</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Environmental Pollutants - toxicity</topic><topic>Female</topic><topic>Fetus - drug effects</topic><topic>Fetus - pathology</topic><topic>filaggrin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Intermediate Filament Proteins - metabolism</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Male</topic><topic>Maternal Exposure - adverse effects</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Morphogenesis - drug effects</topic><topic>Morphogenesis - physiology</topic><topic>mouse</topic><topic>Polychlorinated Dibenzodioxins - toxicity</topic><topic>Pregnancy</topic><topic>Receptors, Aryl Hydrocarbon - genetics</topic><topic>skin</topic><topic>Skin - drug effects</topic><topic>Skin - embryology</topic><topic>Skin - growth & development</topic><topic>TCDD</topic><topic>Teratology. Teratogens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Loertscher, Jennifer A.</creatorcontrib><creatorcontrib>Lin, Tien-Min</creatorcontrib><creatorcontrib>Peterson, Richard E.</creatorcontrib><creatorcontrib>Allen-Hoffmann, B. Lynn</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Loertscher, Jennifer A.</au><au>Lin, Tien-Min</au><au>Peterson, Richard E.</au><au>Allen-Hoffmann, B. Lynn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Accelerated Terminal Differentiation in Fetal Mouse Skin</atitle><jtitle>Toxicological sciences</jtitle><addtitle>Toxicol. Sci</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>68</volume><issue>2</issue><spage>465</spage><epage>472</epage><pages>465-472</pages><issn>1096-6080</issn><issn>1096-0929</issn><eissn>1096-0929</eissn><coden>TOSCF2</coden><abstract>2,3,7,8 Tetrachlorodibenzo-p-dioxin (TCDD), a ubiquitous environmental toxin, has been shown to cause a human skin pathology called chloracne. The majority of laboratory mouse strains, with the exception of mice bearing a mutation in thehairless gene, fail to display overt signs of chloracne upon exposure to TCDD. As a result, only minimal data exist on the effects of TCDD in adult haired mice and no data exist on the effects of TCDD in developing mouse skin. Here we report that TCDD affects the temporal expression of protein markers of keratinocyte terminal differentiation during murine skin morphogenesis. Immunohistochemical analysis of E16 mice reveals accelerated expression of the intermediate filament-associated protein filaggrin in response to TCDD. At a later developmental time and after birth, expression of filaggrin and loricrin is indistinguishable between treatment and control groups. At E16 expression of keratins 5, 6, and 10 are unaltered in TCDD-exposed individuals and TUNEL analysis shows no apoptotic cells in the basal and spinous layers of either treatment or control groups. At E16, immunohistochemical analysis of AhR-null mouse skin reveals accelerated filaggrin expression in both vehicle and TCDD exposed animals. We therefore hypothesize that AhR acts as a modulator of late stage keratinocyte terminal differentiation.</abstract><cop>Cary, NC</cop><pub>Oxford University Press</pub><pmid>12151643</pmid><doi>10.1093/toxsci/68.2.465</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1096-6080 |
ispartof | Toxicological sciences, 2002-08, Vol.68 (2), p.465-472 |
issn | 1096-6080 1096-0929 1096-0929 |
language | eng |
recordid | cdi_proquest_miscellaneous_18679855 |
source | Oxford Journals Online; Free Full-Text Journals in Chemistry |
subjects | AhR Animals Animals, Newborn Biological and medical sciences Biomarkers Embryology: invertebrates and vertebrates. Teratology Environmental Pollutants - toxicity Female Fetus - drug effects Fetus - pathology filaggrin Fundamental and applied biological sciences. Psychology Intermediate Filament Proteins - metabolism Keratinocytes - drug effects Keratinocytes - metabolism Male Maternal Exposure - adverse effects Membrane Proteins - metabolism Mice Mice, Inbred C57BL Mice, Knockout Morphogenesis - drug effects Morphogenesis - physiology mouse Polychlorinated Dibenzodioxins - toxicity Pregnancy Receptors, Aryl Hydrocarbon - genetics skin Skin - drug effects Skin - embryology Skin - growth & development TCDD Teratology. Teratogens |
title | In Utero Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Causes Accelerated Terminal Differentiation in Fetal Mouse Skin |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A30%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20Utero%20Exposure%20to%202,3,7,8-Tetrachlorodibenzo-p-dioxin%20Causes%20Accelerated%20Terminal%20Differentiation%20in%20Fetal%20Mouse%20Skin&rft.jtitle=Toxicological%20sciences&rft.au=Loertscher,%20Jennifer%20A.&rft.date=2002-08-01&rft.volume=68&rft.issue=2&rft.spage=465&rft.epage=472&rft.pages=465-472&rft.issn=1096-6080&rft.eissn=1096-0929&rft.coden=TOSCF2&rft_id=info:doi/10.1093/toxsci/68.2.465&rft_dat=%3Cproquest_cross%3E18679855%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c499t-23562125cc064f3a8bdbed799743881ce49f763461e0516e8ebceae4e19ba4af3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=18679855&rft_id=info:pmid/12151643&rfr_iscdi=true |