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Proteomics reveals major components of oogenesis in the reproductive tract of sugar-fed Anopheles aquasalis
Anopheles (Nyssorhynchus) aquasalis is a malaria vector mainly distributed along the coastal regions of South and Central America. In the absence of an effective vaccine against malaria, strategies for controlling the vector are the main tool for interrupting parasite transmission. Mechanisms of oog...
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Published in: | Parasitology research (1987) 2016-05, Vol.115 (5), p.1977-1989 |
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container_end_page | 1989 |
container_issue | 5 |
container_start_page | 1977 |
container_title | Parasitology research (1987) |
container_volume | 115 |
creator | Dias-Lopes, Geovane Borges-Veloso, Andre Saboia-Vahia, Leonardo Padrón, Gabriel de Faria Castro, Cássia Luana Guimarães, Ana Carolina Ramos Britto, Constança Cuervo, Patricia De Jesus, Jose Batista |
description | Anopheles (Nyssorhynchus) aquasalis is a malaria vector mainly distributed along the coastal regions of South and Central America. In the absence of an effective vaccine against malaria, strategies for controlling the vector are the main tool for interrupting parasite transmission. Mechanisms of oogenesis and embryogenesis in anautogenous mosquitoes are mainly modulated by blood feeding. However, the expression, at the protein level, of genes involved in such mechanisms in sugar-fed females is unknown. In this work, total protein extracts of the reproductive tract of female An. aquasalis that were fed sugar were analyzed using liquid chromatography followed by mass spectrometry for protein identification and bioinformatic tools for data mining. We identified 922 proteins expressed in the organ, and using several databases, we attributed biological meaning for several of them. Remarkably, nine proteins involved in oogenesis were identified in females fed sugar. Putative vitellogenins, vitellogenin receptor, lipid storage droplet, transferrin, ferritin, and apolipoprotein, identified here, are proteins involved in egg development. Proteins involved in embryonic development, such as paxillin, exuperantia, several growth factors, and dorsal switch protein, were identified. Interestingly, in this study, we identified 15 peptidases of various classes such as aminopeptidases, carboxypeptidases, serine protease, cathepsin, and metalloprotease that could potentially interact with male seminal components. Here, we demonstrated that the reproductive tract of female An. aquasalis fed on sugar expresses proteins involved in oogenesis and embryonic development. These findings reveal unknown aspects of the physiology of this organ under the given nutritional conditions. |
doi_str_mv | 10.1007/s00436-016-4940-6 |
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In the absence of an effective vaccine against malaria, strategies for controlling the vector are the main tool for interrupting parasite transmission. Mechanisms of oogenesis and embryogenesis in anautogenous mosquitoes are mainly modulated by blood feeding. However, the expression, at the protein level, of genes involved in such mechanisms in sugar-fed females is unknown. In this work, total protein extracts of the reproductive tract of female An. aquasalis that were fed sugar were analyzed using liquid chromatography followed by mass spectrometry for protein identification and bioinformatic tools for data mining. We identified 922 proteins expressed in the organ, and using several databases, we attributed biological meaning for several of them. Remarkably, nine proteins involved in oogenesis were identified in females fed sugar. Putative vitellogenins, vitellogenin receptor, lipid storage droplet, transferrin, ferritin, and apolipoprotein, identified here, are proteins involved in egg development. Proteins involved in embryonic development, such as paxillin, exuperantia, several growth factors, and dorsal switch protein, were identified. Interestingly, in this study, we identified 15 peptidases of various classes such as aminopeptidases, carboxypeptidases, serine protease, cathepsin, and metalloprotease that could potentially interact with male seminal components. Here, we demonstrated that the reproductive tract of female An. aquasalis fed on sugar expresses proteins involved in oogenesis and embryonic development. These findings reveal unknown aspects of the physiology of this organ under the given nutritional conditions.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-016-4940-6</identifier><identifier>PMID: 26850722</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animal reproduction ; Animals ; Anopheles ; Anopheles - physiology ; Anopheles aquasalis ; Biomedical and Life Sciences ; Biomedicine ; Carbohydrates ; Female ; Gene Expression Regulation - physiology ; Genetic aspects ; Immunology ; Male ; Medical Microbiology ; Methods ; Microbiology ; Nyssorhynchus ; Oogenesis - physiology ; Original Paper ; Proteomics ; Reproduction</subject><ispartof>Parasitology research (1987), 2016-05, Vol.115 (5), p.1977-1989</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>COPYRIGHT 2016 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-39a960976ba61c5c384a678ab9f20034eaf756fd5e7cd8c2c7b18b80ae3e17ce3</citedby><cites>FETCH-LOGICAL-c440t-39a960976ba61c5c384a678ab9f20034eaf756fd5e7cd8c2c7b18b80ae3e17ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26850722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dias-Lopes, Geovane</creatorcontrib><creatorcontrib>Borges-Veloso, Andre</creatorcontrib><creatorcontrib>Saboia-Vahia, Leonardo</creatorcontrib><creatorcontrib>Padrón, Gabriel</creatorcontrib><creatorcontrib>de Faria Castro, Cássia Luana</creatorcontrib><creatorcontrib>Guimarães, Ana Carolina Ramos</creatorcontrib><creatorcontrib>Britto, Constança</creatorcontrib><creatorcontrib>Cuervo, Patricia</creatorcontrib><creatorcontrib>De Jesus, Jose Batista</creatorcontrib><title>Proteomics reveals major components of oogenesis in the reproductive tract of sugar-fed Anopheles aquasalis</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>Anopheles (Nyssorhynchus) aquasalis is a malaria vector mainly distributed along the coastal regions of South and Central America. In the absence of an effective vaccine against malaria, strategies for controlling the vector are the main tool for interrupting parasite transmission. Mechanisms of oogenesis and embryogenesis in anautogenous mosquitoes are mainly modulated by blood feeding. However, the expression, at the protein level, of genes involved in such mechanisms in sugar-fed females is unknown. In this work, total protein extracts of the reproductive tract of female An. aquasalis that were fed sugar were analyzed using liquid chromatography followed by mass spectrometry for protein identification and bioinformatic tools for data mining. We identified 922 proteins expressed in the organ, and using several databases, we attributed biological meaning for several of them. Remarkably, nine proteins involved in oogenesis were identified in females fed sugar. Putative vitellogenins, vitellogenin receptor, lipid storage droplet, transferrin, ferritin, and apolipoprotein, identified here, are proteins involved in egg development. Proteins involved in embryonic development, such as paxillin, exuperantia, several growth factors, and dorsal switch protein, were identified. Interestingly, in this study, we identified 15 peptidases of various classes such as aminopeptidases, carboxypeptidases, serine protease, cathepsin, and metalloprotease that could potentially interact with male seminal components. Here, we demonstrated that the reproductive tract of female An. aquasalis fed on sugar expresses proteins involved in oogenesis and embryonic development. These findings reveal unknown aspects of the physiology of this organ under the given nutritional conditions.</description><subject>Animal reproduction</subject><subject>Animals</subject><subject>Anopheles</subject><subject>Anopheles - physiology</subject><subject>Anopheles aquasalis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Carbohydrates</subject><subject>Female</subject><subject>Gene Expression Regulation - physiology</subject><subject>Genetic aspects</subject><subject>Immunology</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Nyssorhynchus</subject><subject>Oogenesis - physiology</subject><subject>Original Paper</subject><subject>Proteomics</subject><subject>Reproduction</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqFkk-P1SAUxYnROM-nH8CNsnTT8VIo0OXLxD-TTKKJzppQeunwbMsbaCfx28tLR5djWEDgd27uPQdC3jK4ZADqYwYQXFbAZCVaAZV8RnZM8LpibdM8JztoyxkY4xfkVc5HAKakEC_JRS11A6qud-TX9xQXjFNwmSZ8QDtmOtljTNTF6RRnnJdMo6cxDjhjDpmGmS53WOBTiv3qlvCAdEnWLWcsr4NNlceeHuZ4usMRM7X3q812DPk1eeFLfXzzuO_J7edPP6--VjffvlxfHW4qJwQsFW9tK6FVsrOSucZxLaxU2natrwG4QOtVI33foHK9drVTHdOdBoscmXLI9-TDVrd0eL9iXswUssNxtDPGNRumpebAeFv_H1VaqAZEowt6uaGDHdGE2cfz1GX1WNwrTvlQ7g-iAVnsLTHsCdsELsWcE3pzSmGy6bdhYM75mS0_U_Iz5_yMLJp3j_2s3YT9P8XfwApQb0AuT_OAyRzjmuZi55NV328ib6OxQwrZ3P6oC1B-RBlOAP8DIZGvpQ</recordid><startdate>20160501</startdate><enddate>20160501</enddate><creator>Dias-Lopes, Geovane</creator><creator>Borges-Veloso, Andre</creator><creator>Saboia-Vahia, Leonardo</creator><creator>Padrón, Gabriel</creator><creator>de Faria Castro, Cássia Luana</creator><creator>Guimarães, Ana Carolina Ramos</creator><creator>Britto, Constança</creator><creator>Cuervo, Patricia</creator><creator>De Jesus, Jose Batista</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SS</scope><scope>M7N</scope></search><sort><creationdate>20160501</creationdate><title>Proteomics reveals major components of oogenesis in the reproductive tract of sugar-fed Anopheles aquasalis</title><author>Dias-Lopes, Geovane ; Borges-Veloso, Andre ; Saboia-Vahia, Leonardo ; Padrón, Gabriel ; de Faria Castro, Cássia Luana ; Guimarães, Ana Carolina Ramos ; Britto, Constança ; Cuervo, Patricia ; De Jesus, Jose Batista</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-39a960976ba61c5c384a678ab9f20034eaf756fd5e7cd8c2c7b18b80ae3e17ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animal reproduction</topic><topic>Animals</topic><topic>Anopheles</topic><topic>Anopheles - physiology</topic><topic>Anopheles aquasalis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Carbohydrates</topic><topic>Female</topic><topic>Gene Expression Regulation - physiology</topic><topic>Genetic aspects</topic><topic>Immunology</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Nyssorhynchus</topic><topic>Oogenesis - physiology</topic><topic>Original Paper</topic><topic>Proteomics</topic><topic>Reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dias-Lopes, Geovane</creatorcontrib><creatorcontrib>Borges-Veloso, Andre</creatorcontrib><creatorcontrib>Saboia-Vahia, Leonardo</creatorcontrib><creatorcontrib>Padrón, Gabriel</creatorcontrib><creatorcontrib>de Faria Castro, Cássia Luana</creatorcontrib><creatorcontrib>Guimarães, Ana Carolina Ramos</creatorcontrib><creatorcontrib>Britto, Constança</creatorcontrib><creatorcontrib>Cuervo, Patricia</creatorcontrib><creatorcontrib>De Jesus, Jose Batista</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dias-Lopes, Geovane</au><au>Borges-Veloso, Andre</au><au>Saboia-Vahia, Leonardo</au><au>Padrón, Gabriel</au><au>de Faria Castro, Cássia Luana</au><au>Guimarães, Ana Carolina Ramos</au><au>Britto, Constança</au><au>Cuervo, Patricia</au><au>De Jesus, Jose Batista</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomics reveals major components of oogenesis in the reproductive tract of sugar-fed Anopheles aquasalis</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2016-05-01</date><risdate>2016</risdate><volume>115</volume><issue>5</issue><spage>1977</spage><epage>1989</epage><pages>1977-1989</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><abstract>Anopheles (Nyssorhynchus) aquasalis is a malaria vector mainly distributed along the coastal regions of South and Central America. In the absence of an effective vaccine against malaria, strategies for controlling the vector are the main tool for interrupting parasite transmission. Mechanisms of oogenesis and embryogenesis in anautogenous mosquitoes are mainly modulated by blood feeding. However, the expression, at the protein level, of genes involved in such mechanisms in sugar-fed females is unknown. In this work, total protein extracts of the reproductive tract of female An. aquasalis that were fed sugar were analyzed using liquid chromatography followed by mass spectrometry for protein identification and bioinformatic tools for data mining. We identified 922 proteins expressed in the organ, and using several databases, we attributed biological meaning for several of them. Remarkably, nine proteins involved in oogenesis were identified in females fed sugar. Putative vitellogenins, vitellogenin receptor, lipid storage droplet, transferrin, ferritin, and apolipoprotein, identified here, are proteins involved in egg development. Proteins involved in embryonic development, such as paxillin, exuperantia, several growth factors, and dorsal switch protein, were identified. Interestingly, in this study, we identified 15 peptidases of various classes such as aminopeptidases, carboxypeptidases, serine protease, cathepsin, and metalloprotease that could potentially interact with male seminal components. Here, we demonstrated that the reproductive tract of female An. aquasalis fed on sugar expresses proteins involved in oogenesis and embryonic development. These findings reveal unknown aspects of the physiology of this organ under the given nutritional conditions.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>26850722</pmid><doi>10.1007/s00436-016-4940-6</doi><tpages>13</tpages></addata></record> |
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subjects | Animal reproduction Animals Anopheles Anopheles - physiology Anopheles aquasalis Biomedical and Life Sciences Biomedicine Carbohydrates Female Gene Expression Regulation - physiology Genetic aspects Immunology Male Medical Microbiology Methods Microbiology Nyssorhynchus Oogenesis - physiology Original Paper Proteomics Reproduction |
title | Proteomics reveals major components of oogenesis in the reproductive tract of sugar-fed Anopheles aquasalis |
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