Changes in miR-221/222 Levels in Invasive and In Situ Carcinomas of the Breast: Differences in Association with Estrogen Receptor and TIMP3 Expression Levels

Background Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of m...

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Published in:Molecular diagnosis & therapy 2016-12, Vol.20 (6), p.603-615
Main Authors: Petrovic, Nina, Davidovic, Radoslav, Jovanovic-Cupic, Snezana, Krajnovic, Milena, Lukic, Silvana, Petrovic, Milan, Roganovic, Jelena
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cancer
Cancer Research
Cell cycle
Genes
Histology
Human Genetics
Laboratory Medicine
Metastasis
Molecular Medicine
Original Research Article
Pathogenesis
Pathology
Pharmacotherapy
Rodents
Studies
Tumors
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Summary:Background Breast cancer (BC) is a heterogeneous group of diseases that still represents a major cause of death in the female population. MicroRNAs (miRNAs, miRs), such as miR-221 and miR-222, have been shown to be involved in BC pathology by acting via its target genes such as tissue inhibitor of metalloproteinase 3 ( TIMP3 ). Objectives The main goals of this study were to find differences in miR-221/222 levels of expression in BC groups based on invasiveness, and to investigate the association with estrogen receptor (ER), TIMP3 messenger RNA (mRNA) levels, and clinicopathological characteristics of patients and tumors. Methods In this study, we measured levels of miR-221/222 in 63 breast tissue samples by quantitative reverse transcription–polymerase chain reaction (qRT-PCR) using TaqMan ® technology and immunohistochemistry. Results miR-221/222 levels varied significantly across groups based on invasiveness ( P  
ISSN:1177-1062
1179-2000
DOI:10.1007/s40291-016-0230-3