Fumaric Acid Production from Alkali-Pretreated Corncob by Fed-Batch Simultaneous Saccharification and Fermentation Combined with Separated Hydrolysis and Fermentation at High Solids Loading

• Published in: Applied biochemistry and biotechnology 2017-02, Vol.181 (2), p.573-583
• Main Authors: Li, Xin, Zhou, Jin, Ouyang, Shuiping, Ouyang, Jia, Yong, Qiang
• Format: Article
• Language: English
• Subjects: Acid production; Alkalies - chemistry; Batch Cell Culture Techniques - methods; Biochemistry; Bioreactors - microbiology; Biotechnology; Carbohydrate Metabolism - physiology; Chemistry; Chemistry and Materials Science; Fermentation; Fumarates - isolation & purification; Fumarates - metabolism; Glucose; Glucose - metabolism; Hydrolysis; Industrial Waste - prevention & control; Lignin - metabolism; Lignocellulose; Plant Components, Aerial - chemistry; Plant Components, Aerial - microbiology; Rhizopus - metabolism; Rhizopus oryzae; Sodium Hydroxide - chemistry; Solids; Zea mays - chemistry; Zea mays - microbiology
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-016-2232-3

Production of fumaric acid from alkali-pretreated corncob (APC) at high solids loading was investigated using a combination of separated hydrolysis and fermentation (SHF) and fed-batch simultaneous saccharification and fermentation (SSF) by Rhizopus oryzae . Four different fermentation modes were tested to maximize fumaric acid concentration at high solids loading. The highest concentration of 41.32 g/L fumaric acid was obtained from 20 % ( w / v ) APC at 38 °C in the combined SHF and fed-batch SSF process, compared with 19.13 g/L fumaric acid in batch SSF alone. The results indicated that a combination of SHF and fed-batch SSF significantly improved production of fumaric acid from lignocellulose by R. oryzae than that achieved with batch SSF at high solids loading.