Irisolidone attenuates ethanol‐induced gastric injury in mice by inhibiting the infiltration of neutrophils

• Published in: Molecular nutrition & food research 2017-02, Vol.61 (2), p.np-n/a
• Main Authors: Kang, Geum‐Dan, Lee, Sang‐Yoon, Jang, Se‐Eun, Han, Myung Joo, Kim, Dong‐Hyun
• Format: Article
• Language: English
• Subjects: Activation; Activation analysis; Animals; Attenuation; Cell Line; Ethanol; Ethanol - adverse effects; Flavonoids - pharmacology; Gastritis; Gastritis - chemically induced; Gastritis - drug therapy; Gastritis - metabolism; Glycosides - pharmacology; Hemorrhage; Immune system; Infiltration; Injuries; Interleukin 8; Interleukin-8 - secretion; Irisolidone; Isoflavones - pharmacology; Kudzu; Lesions; Leukocytes (neutrophilic); Lipopolysaccharides; Lipopolysaccharides - pharmacology; Male; Mice; Mice, Inbred ICR; Neutrophil; Neutrophils; Neutrophils - drug effects; NF-kappa B - metabolism; NF-κB protein; Peroxidase; Peroxidase - metabolism; Platelet Factor 4 - metabolism; Pretreatment; Protective Agents - pharmacology; Rodents; Stomach; Tumor Necrosis Factor-alpha - secretion
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.201600517

Scope This study was designed to determine whether irisolidone and its glycoside kakkalide, which are the major constituents of the flower of Pueraria lobata (Kudzu) can attenuate ethanol‐induced gastritic injury in mice. Methods and results Irisolidone and kakkalide inhibited IL‐8 secretion and NF‐κB activation in lipopolysaccharide‐stimulated KATO III cells. Therefore, we investigated their protective effects against ethanol‐induced gastric injury in mice. Pretreatment with kakkalide or irisolidone decreased the area of hemorrhagic ulcerative lesions caused by ethanol and suppressed stomach myeloperoxidase activity, CXCL4 secretion, and NF‐κB activation. The ameliorating effect of irisolidone was more potent than that of kakkalide. Conclusion Irisolidone may attenuate ethanol‐induced gastritis by inhibiting the infiltration of immune cells, particularly neutrophils, through the regulation of CXCL‐4 or IL‐8 secretion. In this study, the effect of irisolidone and its glycoside kakkalide isolated from the flower of Pueraria lobata (Kudzu) on the ethanol‐induced gastritic injury in mice. These constituents, particularly irisolidone, attenuated gastritis by inhibiting the infiltration of neutrophils through the regulation of CXCL‐4 or IL‐8 secretion.