Identification, characterization of selenoprotein W and its mRNA expression patterns in response to somatostatin 14, cysteamine hydrochloride, 17β-estradiol and a binary mixture of 17β-estradiol and cysteamine hydrochloride in topmouth culter (Erythroculter ilishaeformis)

• Published in: Fish physiology and biochemistry 2017-02, Vol.43 (1), p.115-126
• Main Authors: Dong, Haiyan, Chen, Wenbo, Sun, Chao, Sun, Jianwei, Wang, Yanlin, Xie, Chao, Fu, Qianwen, Zhu, Junjie, Ye, Jinyun
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Base Sequence; Biomedical and Life Sciences; Brain - metabolism; Cysteamine - pharmacology; DNA, Complementary - genetics; Drug Interactions; Erythroculter ilishaeformis; Estradiol - pharmacology; Female; Freshwater & Marine Ecology; Histology; Life Sciences; Liver - metabolism; Male; Morphology; Ovary - metabolism; Perciformes - genetics; Phylogeny; RNA, Messenger - metabolism; Selenoprotein W - genetics; Somatostatin - pharmacology; Spleen - metabolism; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-016-0272-9

In this study, a selenoprotein W cDNA was cloned from topmouth culter ( Erythroculter ilishaeformis ), and it was designated as EI SelW. The EI SelW open reading frame was composed of 261 base pairs (bp), encoding 86-amino-acid protein. The 5′ untranslated region (UTR) consisted of 104 bp, and the 3′-UTR was composed of 365 bp. A selenocysteine insertion sequence (SECIS) element was found in the 3′-UTR of EI SelW mRNA. The SECIS element was classified as form II because of a small additional apical loop presented in SECIS element of EI SelW mRNA. Bioinformatic approaches showed that the secondary structure of EI SelW was a β1-α1-β2-β3-β4-α2 pattern from amino-terminal to carboxy-terminal. Real-time PCR analysis of EI SelW mRNAs expression in 17 tissues showed that the EI SelW mRNA was predominantly expressed in liver, ovary, pituitary, various regions of the brain, spinal cord and head kidney. Study of intraperitoneal injection showed that the levels of EI SelW mRNA in brain, liver, ovary and spleen were regulated by somatostatin 14 (SS14), 17β-estradiol (E2), cysteamine hydrochloride (CSH) and a binary mixture of E2 and CSH, dependent on the dosage. These results suggest that E2, SS14 and CSH status may affect tissues of selenium metabolism by regulating the expression of SelW mRNA, as SelW plays a central role in selenium metabolism.