Fluoride-Induced Oxidative and Inflammatory Stress in Osteosarcoma Cells: Does It Affect Bone Development Pathway?

Oxidative stress is reported to negatively affect osteoblast cells. Present study reports oxidative and inflammatory signatures in fluoride-exposed human osteosarcoma (HOS) cells, and their possible association with the genes involved in osteoblastic differentiation and bone development pathways. HO...

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Bibliographic Details
Published in:Biological trace element research 2017, Vol.175 (1), p.103-111
Main Authors: Gandhi, Deepa, Naoghare, Pravin K., Bafana, Amit, Kannan, Krishnamurthi, Sivanesan, Saravanadevi
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Bone Development - drug effects
Bones
Cell Line, Tumor
Cells
Fluorides
Fluorides - pharmacology
Gene Expression Regulation, Neoplastic - drug effects
Humans
Inflammation - metabolism
Inflammation - pathology
Inflammatory diseases
Life Sciences
Nutrition
Oncology
Osteosarcoma - metabolism
Osteosarcoma - pathology
Oxidative stress
Oxidative Stress - drug effects
Sodium Fluoride - pharmacology
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Summary:Oxidative stress is reported to negatively affect osteoblast cells. Present study reports oxidative and inflammatory signatures in fluoride-exposed human osteosarcoma (HOS) cells, and their possible association with the genes involved in osteoblastic differentiation and bone development pathways. HOS cells were challenged with sublethal concentration (8 mg/L) of sodium fluoride for 30 days and analyzed for transcriptomic expression. In total, 2632 transcripts associated with several biological processes were found to be differentially expressed. Specifically, genes involved in oxidative stress, inflammation, osteoblastic differentiation, and bone development pathways were found to be significantly altered. Variation in expression of key genes involved in the abovementioned pathways was validated through qPCR. Expression of serum amyloid A1 protein, a key regulator of stress and inflammatory pathways, was validated through western blot analysis. This study provides evidence that chronic oxidative and inflammatory stress may be associated with the fluoride-induced impediment in osteoblast differentiation and bone development.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-016-0756-6