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Effect of gamma radiation on the expression of mRNA growth factors in glycerol cryopreserved human amniotic membrane
Human amniotic membrane (HAM) due to its high biocompatibility, low immunogenicity, anti-microbial, anti-viral properties as well as the presence of growth factors has been used in various clinical applications. The growth factors play an important role in wound healing. The current study aimed to e...
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Published in: | Cell and tissue banking 2016-12, Vol.17 (4), p.643-651 |
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description | Human amniotic membrane (HAM) due to its high biocompatibility, low immunogenicity, anti-microbial, anti-viral properties as well as the presence of growth factors has been used in various clinical applications. The growth factors play an important role in wound healing. The current study aimed to explore the effect of 15 kGy gamma radiation dose on selected growth factors and receptors mRNA present in HAM. Eight growth factors, namely,
EGF
,
HGF
,
KGF
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
,
TGF
-
β3
and
bFGF
and two growth factor receptors,
HGFR
and
KGFR
were evaluated in this study. The total RNA was extracted and converted to complimentary DNA using commercial kits. Subsequently, the mRNA expressions of these growth factors were evaluated using real-time PCR and the results were statistically analyzed using REST-MCS software. This study confirmed the presence of these mRNA growth factors and receptors in fresh, glycerol cryopreserved and irradiated glycerol cryopreserved HAM. In glycerol cryopreserved HAM, the results showed up-regulation of
HGF
and
bFGF
and down-regulation of
EGF
,
HGFR
,
KGF
,
KGFR
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
relative to the fresh HAM which acted as the control, whereas in irradiated glycerol cryopreserved HAM, the results showed up-regulation of
EGF
,
HGF
,
KGF
,
KGFR
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
and down-regulation of
HGFR
,
TGF
-
α
and
bFGF
relative to the glycerol cryopreserved HAM which acted as the control. However, these mRNA expressions did not show any statistical significant difference compared to the control groups. This study concluded that a dose of 15 kGy of gamma radiation did not affect the mRNA expression for the growth factors’ and receptors’ in the glycerol cryopreserved HAM. |
doi_str_mv | 10.1007/s10561-016-9576-3 |
format | article |
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EGF
,
HGF
,
KGF
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
,
TGF
-
β3
and
bFGF
and two growth factor receptors,
HGFR
and
KGFR
were evaluated in this study. The total RNA was extracted and converted to complimentary DNA using commercial kits. Subsequently, the mRNA expressions of these growth factors were evaluated using real-time PCR and the results were statistically analyzed using REST-MCS software. This study confirmed the presence of these mRNA growth factors and receptors in fresh, glycerol cryopreserved and irradiated glycerol cryopreserved HAM. In glycerol cryopreserved HAM, the results showed up-regulation of
HGF
and
bFGF
and down-regulation of
EGF
,
HGFR
,
KGF
,
KGFR
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
relative to the fresh HAM which acted as the control, whereas in irradiated glycerol cryopreserved HAM, the results showed up-regulation of
EGF
,
HGF
,
KGF
,
KGFR
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
and down-regulation of
HGFR
,
TGF
-
α
and
bFGF
relative to the glycerol cryopreserved HAM which acted as the control. However, these mRNA expressions did not show any statistical significant difference compared to the control groups. This study concluded that a dose of 15 kGy of gamma radiation did not affect the mRNA expression for the growth factors’ and receptors’ in the glycerol cryopreserved HAM.</description><identifier>ISSN: 1389-9333</identifier><identifier>EISSN: 1573-6814</identifier><identifier>DOI: 10.1007/s10561-016-9576-3</identifier><identifier>PMID: 27535136</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amnion - drug effects ; Amnion - metabolism ; Amnion - radiation effects ; Biocompatibility ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Cryotherapy ; Female ; Gamma Rays ; Gene expression ; Gene Expression - drug effects ; Gene Expression - radiation effects ; Glycerol ; Glycerol - pharmacology ; Growth factors ; Humans ; Intercellular Signaling Peptides and Proteins - genetics ; Life Sciences ; Polymerase chain reaction ; Receptors, Growth Factor - genetics ; RNA, Messenger - genetics ; Transplant Surgery</subject><ispartof>Cell and tissue banking, 2016-12, Vol.17 (4), p.643-651</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><rights>Cell and Tissue Banking is a copyright of Springer, 2016.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-b297816e54149df8a64411cb85337e4b1342105f21a7014ea165040d3d6d87de3</citedby><cites>FETCH-LOGICAL-c405t-b297816e54149df8a64411cb85337e4b1342105f21a7014ea165040d3d6d87de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27535136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yatim, Rusidah Mat</creatorcontrib><creatorcontrib>Kannan, Thirumulu Ponnuraj</creatorcontrib><creatorcontrib>Ab Hamid, Suzina Sheikh</creatorcontrib><title>Effect of gamma radiation on the expression of mRNA growth factors in glycerol cryopreserved human amniotic membrane</title><title>Cell and tissue banking</title><addtitle>Cell Tissue Bank</addtitle><addtitle>Cell Tissue Bank</addtitle><description>Human amniotic membrane (HAM) due to its high biocompatibility, low immunogenicity, anti-microbial, anti-viral properties as well as the presence of growth factors has been used in various clinical applications. The growth factors play an important role in wound healing. The current study aimed to explore the effect of 15 kGy gamma radiation dose on selected growth factors and receptors mRNA present in HAM. Eight growth factors, namely,
EGF
,
HGF
,
KGF
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
,
TGF
-
β3
and
bFGF
and two growth factor receptors,
HGFR
and
KGFR
were evaluated in this study. The total RNA was extracted and converted to complimentary DNA using commercial kits. Subsequently, the mRNA expressions of these growth factors were evaluated using real-time PCR and the results were statistically analyzed using REST-MCS software. This study confirmed the presence of these mRNA growth factors and receptors in fresh, glycerol cryopreserved and irradiated glycerol cryopreserved HAM. In glycerol cryopreserved HAM, the results showed up-regulation of
HGF
and
bFGF
and down-regulation of
EGF
,
HGFR
,
KGF
,
KGFR
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
relative to the fresh HAM which acted as the control, whereas in irradiated glycerol cryopreserved HAM, the results showed up-regulation of
EGF
,
HGF
,
KGF
,
KGFR
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
and down-regulation of
HGFR
,
TGF
-
α
and
bFGF
relative to the glycerol cryopreserved HAM which acted as the control. However, these mRNA expressions did not show any statistical significant difference compared to the control groups. This study concluded that a dose of 15 kGy of gamma radiation did not affect the mRNA expression for the growth factors’ and receptors’ in the glycerol cryopreserved HAM.</description><subject>Amnion - drug effects</subject><subject>Amnion - metabolism</subject><subject>Amnion - radiation effects</subject><subject>Biocompatibility</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Cryotherapy</subject><subject>Female</subject><subject>Gamma Rays</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression - radiation effects</subject><subject>Glycerol</subject><subject>Glycerol - pharmacology</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Life Sciences</subject><subject>Polymerase chain reaction</subject><subject>Receptors, Growth Factor - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Transplant Surgery</subject><issn>1389-9333</issn><issn>1573-6814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkV1rFDEUhoMotlZ_gDcS8Mab0ZzJ51yWUqtQFESvQyZzsjtlMlmTmer--2a7VUQQhEBC8pw3yXkIeQnsLTCm3xVgUkHDQDWd1Krhj8gpSM0bZUA8rmtuuqbjnJ-QZ6XcMNYy3fKn5KTVkkvg6pQslyGgX2gKdONidDS7YXTLmGZax7JFij93GUu53wk0fvl0Tjc5_Vi2NDi_pFzoONPNtPeY00R93qcDj_kWB7pdo5upi_OYltHTiLHPbsbn5ElwU8EXD_MZ-fb-8uvFh-b689XHi_Prxgsml6ZvO21AoRQguiEYp4QA8L2RnGsUPXDR1g6EFpxmINCBkkywgQ9qMHpAfkbeHHN3OX1fsSw2jsXjNNU3pLVYMMrwtpOg_wMVoDsjhKno67_Qm7TmuX7knuJQm3ug4Ej5nErJGOwuj9HlvQVmD_bs0Z6t9uzBnuW15tVD8tpHHH5X_NJVgfYIlHo0bzD_cfU_U-8AwS2j5A</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Yatim, Rusidah Mat</creator><creator>Kannan, Thirumulu Ponnuraj</creator><creator>Ab Hamid, Suzina Sheikh</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20161201</creationdate><title>Effect of gamma radiation on the expression of mRNA growth factors in glycerol cryopreserved human amniotic membrane</title><author>Yatim, Rusidah Mat ; Kannan, Thirumulu Ponnuraj ; Ab Hamid, Suzina Sheikh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-b297816e54149df8a64411cb85337e4b1342105f21a7014ea165040d3d6d87de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Amnion - drug effects</topic><topic>Amnion - metabolism</topic><topic>Amnion - radiation effects</topic><topic>Biocompatibility</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Cryotherapy</topic><topic>Female</topic><topic>Gamma Rays</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression - radiation effects</topic><topic>Glycerol</topic><topic>Glycerol - pharmacology</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Life Sciences</topic><topic>Polymerase chain reaction</topic><topic>Receptors, Growth Factor - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Transplant Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yatim, Rusidah Mat</creatorcontrib><creatorcontrib>Kannan, Thirumulu Ponnuraj</creatorcontrib><creatorcontrib>Ab Hamid, Suzina Sheikh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (ProQuest Medical & Health Databases)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cell and tissue banking</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yatim, Rusidah Mat</au><au>Kannan, Thirumulu Ponnuraj</au><au>Ab Hamid, Suzina Sheikh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of gamma radiation on the expression of mRNA growth factors in glycerol cryopreserved human amniotic membrane</atitle><jtitle>Cell and tissue banking</jtitle><stitle>Cell Tissue Bank</stitle><addtitle>Cell Tissue Bank</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>17</volume><issue>4</issue><spage>643</spage><epage>651</epage><pages>643-651</pages><issn>1389-9333</issn><eissn>1573-6814</eissn><abstract>Human amniotic membrane (HAM) due to its high biocompatibility, low immunogenicity, anti-microbial, anti-viral properties as well as the presence of growth factors has been used in various clinical applications. The growth factors play an important role in wound healing. The current study aimed to explore the effect of 15 kGy gamma radiation dose on selected growth factors and receptors mRNA present in HAM. Eight growth factors, namely,
EGF
,
HGF
,
KGF
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
,
TGF
-
β3
and
bFGF
and two growth factor receptors,
HGFR
and
KGFR
were evaluated in this study. The total RNA was extracted and converted to complimentary DNA using commercial kits. Subsequently, the mRNA expressions of these growth factors were evaluated using real-time PCR and the results were statistically analyzed using REST-MCS software. This study confirmed the presence of these mRNA growth factors and receptors in fresh, glycerol cryopreserved and irradiated glycerol cryopreserved HAM. In glycerol cryopreserved HAM, the results showed up-regulation of
HGF
and
bFGF
and down-regulation of
EGF
,
HGFR
,
KGF
,
KGFR
,
TGF
-
α
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
relative to the fresh HAM which acted as the control, whereas in irradiated glycerol cryopreserved HAM, the results showed up-regulation of
EGF
,
HGF
,
KGF
,
KGFR
,
TGF
-
β1
,
TGF
-
β2
and
TGF
-
β3
and down-regulation of
HGFR
,
TGF
-
α
and
bFGF
relative to the glycerol cryopreserved HAM which acted as the control. However, these mRNA expressions did not show any statistical significant difference compared to the control groups. This study concluded that a dose of 15 kGy of gamma radiation did not affect the mRNA expression for the growth factors’ and receptors’ in the glycerol cryopreserved HAM.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>27535136</pmid><doi>10.1007/s10561-016-9576-3</doi><tpages>9</tpages></addata></record> |
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subjects | Amnion - drug effects Amnion - metabolism Amnion - radiation effects Biocompatibility Biomedical and Life Sciences Biomedicine Cell Biology Cryopreservation - methods Cryoprotective Agents - pharmacology Cryotherapy Female Gamma Rays Gene expression Gene Expression - drug effects Gene Expression - radiation effects Glycerol Glycerol - pharmacology Growth factors Humans Intercellular Signaling Peptides and Proteins - genetics Life Sciences Polymerase chain reaction Receptors, Growth Factor - genetics RNA, Messenger - genetics Transplant Surgery |
title | Effect of gamma radiation on the expression of mRNA growth factors in glycerol cryopreserved human amniotic membrane |
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