Susceptibility of a number of Australian freshwater fishes to dwarf gourami iridovirus (Infectious spleen and kidney necrosis virus)

• Published in: Journal of fish diseases 2017-03, Vol.40 (3), p.293-310
• Main Authors: Rimmer, A E, Whittington, R J, Tweedie, A, Becker, J A
• Format: Article
• Language: English
• Subjects: Animals; Australia; Catfishes; conservation; Disease Susceptibility - veterinary; Disease Susceptibility - virology; DNA Virus Infections - transmission; DNA Virus Infections - veterinary; DNA Virus Infections - virology; experimental trial; Fish Diseases - transmission; Fish Diseases - virology; Fresh Water; Freshwater; Infectious spleen and kidney necrosis virus; Iridoviridae; Iridoviridae - physiology; iridovirus; Maccullochella peelii; Macquaria ambigua; Macquaria australasica; megalocytivirus; Nannoperca australis; Perciformes; susceptibility; Tandanus tandanus
• ISSN: 0140-7775; 1365-2761
• DOI: 10.1111/jfd.12510

Megalocytiviruses cause high mortality diseases that have seriously impacted aquaculture, with the most frequent outbreaks occurring in East and South‐East Asia. The international trade of juvenile fish for food and ornamental aquaculture has aided the spread of these viruses, which have spread to Europe and Australia and other regions. Australian freshwater fishes were examined for susceptibility to infection with the exotic megalocytivirus, dwarf gourami iridovirus (DGIV), which belongs to a group with the type species, Infectious spleen and kidney necrosis virus (ISKNV). Fish were held at 23 ± 1 °C and challenged by intraperitoneal (IP) injection or by cohabitation with Murray cod, Maccullochella peelii (Mitchell) infected with DGIV. A species was deemed to be susceptible to DGIV based on evidence of viral replication, as determined by qPCR, and megalocytic inclusion bodies observed histologically. Horizontal transmission occurred between infected Murray cod and golden perch, Macquaria ambigua (Richardson), Macquarie perch, Macquaria australasica (Cuvier) and Murray cod. This indicated that DGIV shed from infected fish held at 23 °C can survive in fresh water and subsequently infect these naïve fish. Further, DGIV administered IP was highly pathogenic to golden perch, Macquarie perch and Murray cod. Compared to these species, the susceptibility of southern pygmy perch, Nannoperca australis (Gunther) was lower. Freshwater catfish (dewfish), Tandanus tandanus (Mitchell), were not susceptible under the experimental conditions based on the absence of clinical disease, mortality and virus replication. This study showed the potential risks associated with naïve and DGIV‐infected fish sharing a common water source.