Real-time imaging of the growth-inhibitory effect of JS399-19 on Fusarium

Real-time imaging was used to study the effects of a novel Fusarium-specific cyanoacrylate fungicide (JS399-19) on growth and morphology of four Fusarium sp. This fungicide targets the motor domain of type I myosin. Fusarium graminearum PH-1, Fusarium solani f. sp. pisi 77-13-4, Fusarium avenaceum I...

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Bibliographic Details
Published in:Pesticide biochemistry and physiology 2016-11, Vol.134, p.24-30
Main Authors: Wollenberg, Rasmus D., Donau, Søren S., Nielsen, Thorbjørn T., Sørensen, Jens L., Giese, Henriette, Wimmer, Reinhard, Søndergaard, Teis E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - pharmacology
Antimicrobial susceptibility testing
Cyanoacrylates
Drug Resistance, Fungal - genetics
Fungal Proteins - chemistry
Fungicide resistance
Fungicides
Fungicides, Industrial - pharmacology
Fusarium
Fusarium - cytology
Fusarium - drug effects
Fusarium - genetics
Fusarium - growth & development
Fusarium avenaceum
Fusarium graminearum
Fusarium solani
Hyphae - drug effects
Hyphae - growth & development
Microscopy
Myosin Type I - chemistry
Phenylpropionates - pharmacology
Real-time microscopy
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Summary:Real-time imaging was used to study the effects of a novel Fusarium-specific cyanoacrylate fungicide (JS399-19) on growth and morphology of four Fusarium sp. This fungicide targets the motor domain of type I myosin. Fusarium graminearum PH-1, Fusarium solani f. sp. pisi 77-13-4, Fusarium avenaceum IBT8464, and Fusarium avenaceum 05001, which has a K216Q amino-acid substitution at the resistance-implicated site in its myosin type I motor domain, were analyzed. Real-time imaging shows that JS399-19 inhibits fungal growth but not to the extent previously reported. The fungicide causes the hypha to become entangled and unable to extend vertically. This implies that type I myosin in Fusarium is essential for hyphal and mycelia propagation. The K216Q substitution correlates with reduced susceptibility in F. avenaceum. [Display omitted] •High-throughput real-time microscopy is used for JS399-19-susceptibility testing of Fusarium fungi.•Growth of JS399-19 susceptible fungi are less affected by JS399-19 than previously recognized.•The use of JS399-19 as a fungicide calls for proper pesticide management and resistance monitoring.
ISSN:0048-3575
1095-9939
DOI:10.1016/j.pestbp.2016.05.002