Extraction and electrophoretic analysis of large dsRNAs from desiccated plant tissues infected with plant viruses and biotrophic fungi

Extraction and electrophoretic analysis of large dsRNAs from plant and fungal tissues has been used successfully to detect RNA viruses infecting plants, fungi, and oomycetes. We modified a previously reported dsRNA extraction protocol and used it to detect a wide variety of plant and putative fungal...

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Bibliographic Details
Published in:European journal of plant pathology 2017-02, Vol.147 (2), p.431-441
Main Authors: Khankhum, S., Escalante, C., de Souto and, E. Rodrigues, Valverde, R. A.
Format: Article
Language:English
Subjects:
Agriculture
Airborne microorganisms
Biomedical and Life Sciences
Ecology
Fungi
Life Sciences
Oomycetes
Plant diseases
Plant extracts
Plant Pathology
Plant Sciences
Plant tissues
Ribonucleic acid
RNA
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Summary:Extraction and electrophoretic analysis of large dsRNAs from plant and fungal tissues has been used successfully to detect RNA viruses infecting plants, fungi, and oomycetes. We modified a previously reported dsRNA extraction protocol and used it to detect a wide variety of plant and putative fungal viruses from infected plant tissues. The modified protocol was used successfully to extract large dsRNAs from 50 to 70 mg of desiccated plant tissues infected with acute and persistent RNA viruses and from plant tissues infected with biotrophic fungi causing rusts and powdery mildew diseases. The protocol proved to be efficient, fast, economic and versatile and requires relatively small amounts of desiccated tissue.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-016-1014-7