PCR fishing for red endophytes in British Columbia Kallymeniaceae (Gigartinales, Florideophyceae) uncovers the species‐rich Kallymenicola gen. nov. (Palmariales, Nemaliophycidae)

Unexpected contaminants uncovered during routine COI‐5P DNA barcoding of British Columbia Kallymeniaceae indicated the presence of a novel lineage allied to the family Meiodiscaceae, Palmariales. Available rbcL data for species of this family were used to design specific primers to screen for the pr...

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Bibliographic Details
Published in:Journal of phycology 2017-06, Vol.53 (3), p.577-588
Main Authors: Evans, Joshua R., Saunders, Gary W., Müller, K.
Format: Article
Language:English
Subjects:
Biogeography
British Columbia
COI‐5P
Contaminants
Deoxyribonucleic acid
DNA
DNA barcoding
DNA Barcoding, Taxonomic
Endophytes
Endophytes - classification
Endophytes - genetics
Fishing
Gene sequencing
Host alternation
Host specificity
Kallymeniaceae
Kallymenicola
Meiodiscaceae
New genera
New species
Nucleotide sequence
Palmariales
PCR
Polymerase Chain Reaction
Primers
Products
rbcL
Rhodophyta - classification
Rhodophyta - genetics
Ribulose-Bisphosphate Carboxylase - genetics
Species
Specificity
Switching
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Summary:Unexpected contaminants uncovered during routine COI‐5P DNA barcoding of British Columbia Kallymeniaceae indicated the presence of a novel lineage allied to the family Meiodiscaceae, Palmariales. Available rbcL data for species of this family were used to design specific primers to screen for the presence of the meiodiscacean species in 534 kallymeniacean specimens primarily from British Columbia, Canada. Ultimately, 43 positive PCR products representing six diverse genetic groups from nine host species were uncovered; three are described here in the new genus Kallymenicola gen. nov., viz., K. invisiblis sp. nov., K penetrans sp. nov., and K superficialis sp. nov. Although genetic groups loosely displayed evidence of host specificity and cospeciation, examples of host switching with interesting biogeographical patterns were also documented.
ISSN:0022-3646
1529-8817
DOI:10.1111/jpy.12522