CRYOPRESERVATION OF SOUR ORANGE (CITRUS AURANTIUM L.) SHOOT TIPS

The objective of this study was to establish a cryopreservation protocol for sour orange (Citrus aurantium L.). Cryopreservation was carried out via encapsulation— dehydration, vitrification, and encapsulation— vitrification on shoot tips excised from in vitro cultures. Results indicated that a maxi...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2002-11, Vol.38 (6), p.602-607
Main Authors: AL-ABABNEH, SAMIA S., KARAM, NABILA S., SHIBU, RIDA A.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Cryopreservation
Dehydration
Encapsulating
Encapsulation
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetic resources, diversity
Genetics and breeding of economic plants
Liquid nitrogen
MICROPROPAGATION
Moisture content
Plant cells
Plant material
Regrowth
Silica gel
Vitrification
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Summary:The objective of this study was to establish a cryopreservation protocol for sour orange (Citrus aurantium L.). Cryopreservation was carried out via encapsulation— dehydration, vitrification, and encapsulation— vitrification on shoot tips excised from in vitro cultures. Results indicated that a maximum of 83% survival and 47% regrowth of encapsulateddehydrated and cryopreserved shoot tips was obtained with 0.5 M sucrose in the preculture medium and further dehydration for 6 h to attain 18% moisture content. Dehydration of encapsulated shoot tips with silica gel for 2 h resulted in 93% survival but only 37% regrowth of cryopreserved shoot tips. After preculturing with 0.5 M sucrose, 80% of the vitrified cryopreserved shoots survived when 2 M sucrose plus 10% dimethyl sulfoxide (DMSO) was used as a cryoprotectant for 20min at 25°C. Survival and regrowth of vitrified cryopreserved shoot tips were 67% and 43%, respectively, when 0.4 M sucrose plus 2 M glycerol was used as a loading solution followed by application of 100% plant vitrification solution (PVS2) for 20 min. Increased duration of exposure to the loading solution up to 60 min increased survival (83%) and regrowth (47%) of cryopreserved shoot tips. With encapsulation-vitrification, dehydration with 100% PVS2 for 2 or 3 h at 0°C resulted in 50 or 57% survival and 30 or 40% regrowth, respectively, of cryopreserved shoot tips.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2002349