Determination of Zn in Dry Feeds for Cats and Dogs by Energy-Dispersive X-Ray Fluorescence Spectrometry

• Published in: Journal of AOAC International 2016-11, Vol.99 (6), p.1572-1575
• Main Authors: Ávila, Dayara Virgnía L, Souza, Sidnei O, Costa, Silvânio Silvério L, Araujo, Rennan Geovanny O, Garcia, Carlos Alexandre B, Alves, José do Patrocínio H, Passos, Elisangela A
• Format: Article
• Language: English
• Subjects: Animal Feed - analysis; Animal feeding and feeds; Animals; Cats; Chemical properties; Dietary Fiber - analysis; Dogs; Fluorescence spectroscopy; Identification and classification; Methods; Nutritional aspects; Spectrometry, X-Ray Emission; X-ray spectroscopy; Zinc (Nutrient); Zinc - analysis
• ISSN: 1060-3271; 1944-7922
• DOI: 10.5740/jaoacint.16-0105

This work describes an analytical method for Zn determination in dry feeds for cats and dogs by energy-dispersive X-ray fluorescence (EDXRF). Samples of dry feed were powdered and prepared in the form of pellets for direct analysis by EDXRF. The LOQ (10σ) was 0.4 mg/kg. The samples were also analyzed by inductively coupled plasma-optical emission spectrometry (ICP-OES) as an independent comparative method. Application of a paired t-test showed no significant differences between Zn concentrations obtained by EDXRF and ICP-OES (at a 95% confidence level). Analysis of variance was also applied to the results and revealed no significant differences between the two techniques (at a 95% confidence level). The precision, expressed as the RSD (n = 3), was RSD < 4.55%. This analytical method provides a simple, rapid, accurate, and precise determination of Zn in dry feeds for cats and dogs by EDXRF as direct, solid-sample analysis.