Reduced immunohistochemical expression of adhesion molecules in vitiligo skin biopsies

• Published in: Pathology, research and practice research and practice, 2017-03, Vol.213 (3), p.199-204
• Main Authors: Reichert Faria, Adriane, Jung, Juliana Elizabeth, Silva de Castro, Caio César, de Noronha, Lucia
• Format: Article
• Language: English
• Subjects: Adhesion; Adult; beta Catenin - metabolism; Biopsy; Cadherins - metabolism; Cell Adhesion Molecules - metabolism; Collagen Type IV - metabolism; Female; Humans; Immunohistochemistry; Integrins - metabolism; Intercellular Adhesion Molecule-1 - metabolism; Laminin - metabolism; Male; Middle Aged; Paraffin- embedded; Skin - metabolism; Skin - pathology; Skin biopsy; Vascular Cell Adhesion Molecule-1 - metabolism; Vitiligo; Vitiligo - metabolism; Vitiligo - pathology
• ISSN: 0344-0338; 1618-0631
• DOI: 10.1016/j.prp.2016.12.019

Because defects in adhesion impairment seem to be involved in the etiopathogenesis of vitiligo, this study aimed to compare the immunohistochemical expression of several adhesion molecules in the epidermis of vitiligo and non lesional vitiligo skin. Sixty-six specimens of lesional and non lesional skin from 33 volunteers with vitiligo were evaluated by immunohistochemistry using anti-beta-catenin, anti-E-cadherin, anti-laminin, anti-beta1 integrin, anti-collagen IV, anti-ICAM-1 and anti-VCAM-1 antibodies. Biopsies of vitiligo skin demonstrated a significant reduction in the expression of laminin and integrin. The average value of the immunohistochemically positive reaction area of the vitiligo specimens was 3053.2μm2, compared with the observed value of 3431.8μm2 in non vitiligo skin (p=0.003) for laminin. The immuno-positive area was 7174.6μm2 (vitiligo) and 8966.7μm2 (non lesional skin) for integrin (p=0.042). A reduction in ICAM-1 and VCAM-1 expression in the basal layer of the epidermis in vitiligo samples was also observed (p=0.001 and p