First isolation and genetic characteristics of porcine sapeloviruses in Hunan, China

Outbreaks of diarrhea in piglets cause serious economic consequences in China. Diarrhetic fecal samples from 20 Hunan farm piglets were tested and found to be positive for porcine epidemic diarrhea virus (PEDV) by RT-PCR, although incubation with porcine kidney (PK-15) cells failed to produce infect...

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Bibliographic Details
Published in:Archives of virology 2017-06, Vol.162 (6), p.1589-1597
Main Authors: Yang, Taotao, Li, Runcheng, Peng, Wang, Ge, Meng, Luo, Binyu, Qu, Tailong, Yu, Xinglong
Format: Article
Language:English
Subjects:
Amino acid sequence
Animals
Biomedical and Life Sciences
Biomedicine
Capsid Proteins - genetics
China - epidemiology
Coronavirus Infections - epidemiology
Coronavirus Infections - veterinary
Coronavirus Infections - virology
Diarrhea
Disease Outbreaks
Feces - virology
Genes
Genetic diversity
Genetic Variation
Genome, Viral
Homology
Infectious Diseases
Medical Microbiology
Nucleotide sequence
Open reading frames
Original Article
Outbreaks
Phylogeny
Polymerase chain reaction
Polyproteins
Porcine epidemic diarrhea virus - genetics
Porcine epidemic diarrhea virus - isolation & purification
Recombination
Recombination, Genetic
Sequence Analysis, DNA
Swine
Swine Diseases - epidemiology
Swine Diseases - virology
Transmissible gastroenteritis
Virology
VP1 protein
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Summary:Outbreaks of diarrhea in piglets cause serious economic consequences in China. Diarrhetic fecal samples from 20 Hunan farm piglets were tested and found to be positive for porcine epidemic diarrhea virus (PEDV) by RT-PCR, although incubation with porcine kidney (PK-15) cells failed to produce infectious PEDV. Four porcine sapelovirus (PSV) strains (designated as PSV-HuNs) were isolated from four of the samples. Genomic sequence analysis revealed open reading frames encoding polyproteins of 2,331 (HuN1, 2 and 3) and 2,332 (HuN4) amino acids. Homology comparisons of the VP1 gene of the four Hunan strains with previously reported PSV strains revealed nucleotide sequence identities ranging from 74.2 to 98.6%, and deduced amino acid sequence identities from 79.5 to 98%. Phylogenetic analyses based on full-length and partial VP1 gene sequences showed that 3 of the PSV-HuN strains (HuN2, 3 and 4) clustered within a clade distinct from HuN1 as well as from all PSVs previously isolated in China, thereby showing that genetic diversity exists within Chinese PSVs. In addition, recombination analysis among PSVs indicates that a recombinant (HuN2 strain) exist in China.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-017-3264-x