Internal Dynamics of the 3‑Pyrroline‑N‑Oxide Ring in Spin-Labeled Proteins

Site-directed spin labeling is a versatile tool to study structure as well as dynamics of proteins using EPR spectroscopy. Methanethiosulfonate (MTS) spin labels tethered through a disulfide linkage to an engineered cysteine residue were used in a large number of studies to extract structural as wel...

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Bibliographic Details
Published in:The journal of physical chemistry letters 2017-03, Vol.8 (6), p.1113-1117
Main Authors: Consentius, Philipp, Loll, Bernhard, Gohlke, Ulrich, Alings, Claudia, Müller, Carsten, Müller, Robert, Teutloff, Christian, Heinemann, Udo, Kaupp, Martin, Wahl, Markus C, Risse, Thomas
Format: Article
Language:English
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Summary:Site-directed spin labeling is a versatile tool to study structure as well as dynamics of proteins using EPR spectroscopy. Methanethiosulfonate (MTS) spin labels tethered through a disulfide linkage to an engineered cysteine residue were used in a large number of studies to extract structural as well as dynamic information on the protein from the rotational dynamics of the nitroxide moiety. The ring itself was always considered to be a rigid body. In this contribution, we present a combination of high-resolution X-ray crystallography and EPR spectroscopy of spin-labeled protein single crystals demonstrating that the nitroxide ring inverts fast at ambient temperature while exhibiting nonplanar conformations at low temperature. We have used quantum chemical calculations to explore the potential energy that determines the ring dynamics as well as the impact of the geometry on the magnetic parameters probed by EPR spectroscopy.
ISSN:1948-7185
1948-7185
DOI:10.1021/acs.jpclett.6b02971