Lipidomics in research on yeast membrane lipid homeostasis

Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors addressing the dynamics of lipid metabolism. Here m...

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Bibliographic Details
Published in:Biochimica et biophysica acta. Molecular and cell biology of lipids 2017-08, Vol.1862 (8), p.797-799
Main Author: de Kroon, Anton I.P.M.
Format: Article
Language:English
Subjects:
Homeostasis - physiology
Isotope Labeling - methods
Lipid Metabolism - physiology
Lipidomics
Lipids - chemistry
Mass Spectrometry - methods
Membrane lipid composition
Membrane Lipids - metabolism
Membranes - metabolism
Metabolomics - methods
Stable isotope labeling
Yeast lipids
Yeasts - metabolism
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Summary:Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors addressing the dynamics of lipid metabolism. Here my experience with, and view on mass spectrometry-based lipid analysis is presented, with emphasis on aspects of quantification of membrane lipid composition of the yeast Saccharomyces cerevisiae. This article is part of a Special Issue entitled: BBALIP_Lipidomics Opinion Articles edited by Sepp Kohlwein. •Mass spectrometry is increasingly used for studying the yeast lipidome.•An opinion is presented on (dynamic) lipidomics approaches in yeast.•Aspects of quantification of lipidomics data are emphasized.
ISSN:1388-1981
1879-2618
1879-2618
DOI:10.1016/j.bbalip.2017.02.007