Extraction of Amphetamine and Methamphetamine from Urine Specimens with Cerex Polycrom™ Clin II Solid-Phase Extraction Columns and the Speedisk™ 48 Pressure Processor

Extraction of amphetamine and methamphetamine in urine was investigated using Cerex Polycrom Clin II solid-phase extraction columns and the Speedisk 48 Pressure Processor as a replacement for our liquid-liquid procedure. Linearity for urine standards extracted with the Cerex-Speedisk method ranged f...

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Bibliographic Details
Published in:Journal of analytical toxicology 2002-09, Vol.26 (6), p.347-354
Main Authors: Churley, Melissa, Robandt, Paul V., Kuhnle, Judith A., Lyons, Timothy P., Bruins, Mark R.
Format: Article
Language:English
Subjects:
Amphetamine - urine
Analysis
Biological and medical sciences
Calibration
Chemistry Techniques, Analytical - methods
Drug addictions
Gas Chromatography-Mass Spectrometry
General pharmacology
Hazardous Waste
Humans
Medical sciences
Methamphetamine - urine
Pharmacology. Drug treatments
Polymers
Pressure
Reproducibility of Results
Sensitivity and Specificity
Toxicology
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Summary:Extraction of amphetamine and methamphetamine in urine was investigated using Cerex Polycrom Clin II solid-phase extraction columns and the Speedisk 48 Pressure Processor as a replacement for our liquid-liquid procedure. Linearity for urine standards extracted with the Cerex-Speedisk method ranged from 50 ng/mL for methamphetamine and from 150 ng/mL for amphetamine to 10,000 ng/mL for both. The mean recovery at the 500-ng/mL cutoff for three different lots of columns was 96.4% for AMP and 95.7% for MET. The mean of the within-run means for three batches was 495.4 ng/mL with a coefficient of variation (CV) of 1.2% or less for amphetamine and 496.4 ng/mL for methamphetamine with a CV of 1.7% or less. Thirty-six specimens containing amphetamine and the same number for methamphetamine were analyzed by both the Cerex-Speedisk and liquid-liquid methods. The correlation for specimens containing amphetamine gave an r2 of 0.9986 with a slope of 0.99; for methamphetamine, the r2 was 0.9997 with a slope of 0.98. The Cerex-Speedisk method cut extraction time in half, was less costly, and greatly reduced the volume of hazardous waste.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/26.6.347