Differentiation of Xanthomonas species pathogenic to sugarcane by PCR-RFLP analysis

The PCR-RFLP of the 16S-23S rDNA spacer region was used to differentiate Xanthomonas species pathogenic to sugarcane. Strains of X. albilineans, X. campestris pv. vasculorum Types A and B, X. sacchari and Xanthomonas sp. from Trinidad, South Africa and India were examined. The amplification products...

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Bibliographic Details
Published in:European journal of plant pathology 2003-03, Vol.109 (3), p.283-288
Main Authors: DESTEFANO, S. A. L, ALMEIDA, I. M. G, RODRIGUES, J. NETO, FERREIRA, M, BALANI, D. M
Format: Article
Language:English
Subjects:
Bacteria
Bacterial plant pathogens
Biological and medical sciences
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Generalities. Techniques
Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control
Microbiology
Phytopathology. Animal pests. Plant and forest protection
Sugarcane
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Summary:The PCR-RFLP of the 16S-23S rDNA spacer region was used to differentiate Xanthomonas species pathogenic to sugarcane. Strains of X. albilineans, X. campestris pv. vasculorum Types A and B, X. sacchari and Xanthomonas sp. from Trinidad, South Africa and India were examined. The amplification products were digested with Alu I, Hae III, Hpa II and Mbo I and the results showed that the different groups of bacterial strains exhibited distinct RFLP patterns for each tested endonuclease, except X. albilineans and X. sacchari which could only be differentiated from each other by the digestion with Hpa II. The results also allowed the separation of X.c. pv. vasculorum Type A from X.c. pv. vasculorum Type B and strongly suggested that the analyzed Xanthomonas sp. strains belong to X. sacchari. Nine X. campestris (pv. not determined) strains included in this study showed identical profiles to X.c. pv. vasculorum Type A group and DNA-DNA hybridization experiments confirmed these results. PCR-RFLP of the 16S-23S rDNA spacer region could be applied as a reliable method for differentiating the xanthomonads pathogenic to sugarcane.[PUBLICATION ABSTRACT]
ISSN:0929-1873
1573-8469
DOI:10.1023/A:1022894907176