Automated processing, extraction and detection of herpes simplex virus types 1 and 2: A comparative evaluation of three commercial platforms using clinical specimens

Highlights • This is the first study to compare the performance of three, commercially-available automated systems for the detection and differentiation of HSV types 1 and 2 from clinical specimens. • This study utilized a consensus reference standard, defined as the result achieved by at least 3 of...

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Bibliographic Details
Published in:Journal of clinical virology 2017-04, Vol.89, p.30-33
Main Authors: Binnicker, Matthew J, Espy, Mark J, Duresko, Brian, Irish, Cole, Mandrekar, Jay
Format: Article
Language:English
Subjects:
Allergy and Immunology
Automated
Automation, Laboratory - methods
Dermal
DNA, Viral - analysis
DNA, Viral - genetics
DNA, Viral - isolation & purification
Female
Genital
Herpes Genitalis - diagnosis
Herpes Simplex - diagnosis
Herpesvirus 1, Human - isolation & purification
Herpesvirus 2, Human - isolation & purification
HSV
Humans
Infectious Disease
Male
Molecular Diagnostic Techniques - methods
Nucleic acid amplification tests
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Summary:Highlights • This is the first study to compare the performance of three, commercially-available automated systems for the detection and differentiation of HSV types 1 and 2 from clinical specimens. • This study utilized a consensus reference standard, defined as the result achieved by at least 3 of the 4 molecular methods. This is advantageous over past studies that have used viral culture as a reference standard, which typically underestimates the specificity of molecular methods.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2017.02.006