Mandevilla longiflora (Desf.) Pichon improves airway inflammation in a murine model of allergic asthma

• Published in: Journal of ethnopharmacology 2017-03, Vol.200, p.51-59
• Main Authors: de Almeida, Danielle Ayr Tavares, Rosa, Suellen Iara Guirra, da Cruz, Thais Campos Dias, Pavan, Eduarda, Sabino Damazo, Amílcar, Soares, Ilsamar Mendes, Ascêncio, Sérgio Donizeti, Macho, Antonio, Martins, Domingos Tabajara de Oliveira
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - therapeutic use; Apocynaceae; Asthma; Asthma - drug therapy; Asthma - immunology; Asthma - pathology; Bronchial Hyperreactivity - chemically induced; Bronchial Hyperreactivity - drug therapy; Bronchial Hyperreactivity - immunology; Bronchoalveolar Lavage Fluid - immunology; Inflammation; Inflammation - chemically induced; Inflammation - drug therapy; Inflammation - immunology; Male; Mandevilla longiflora; Mice; Mice, Inbred BALB C; Ovalbumin - toxicity; Plant Extracts - isolation & purification; Plant Extracts - therapeutic use
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2017.02.015

Mandevilla longiflora, popularly known as “velame" in central Brazil, is a subshrub widely distributed in South America. Its xylopodium is used in the form of a decoction or infusion to treat inflammation and other ailments. This study aimed to evaluate the anti-inflammatory potential of M. longiflora in an in vivo model of ovalbumin-induced immediate hypersensitivity, identifying its effects on leukocyte infiltration, IgE and LTB4 levels, and Th2 cytokine production. In addition, HPLC fingerprint of the extract was performed. The hydroethanolic extract 70% of M. longiflora (HEMI) was obtained by maceration of the plant xylopodium. Swiss mice were sensitized by i.p. injection OVA-aluminium hydroxide on days 1 and 10. Nine days after the last sensitisation animals were challenged for 6 consecutive days with OVA solution for 20min daily in a closed chamber under continuous flow of aerosol. The animals were treated with HEMl (20, 50 and 200mg/kg p.o.), 2 times per day, and euthanized 24h later. Animals treated with vehicle (2% Tween-20) or dexamethasone were used as negative and positive controls, respectively. The recruitment of inflammatory cells into the pulmonary cavity was evaluated by counting cells present in broncho-alveolar lavage fluid (BALF). Lung tissue was also collected for histopathology and infiltration analysis. Quantification of IL-4, IL-5 and IL-13 from the BALF, and IgE, and LTB4 from plasma, were conducted by immunoassay. The HEMl attenuated leukocyte migration into the airways, which was evidenced by a decrease in eosinophils, neutrophils and mononuclear cells, both in BALF quantification and by histopathological analysis, as well as decreasing the concentrations of IL-4, IL-5, IL-13, IgE and LTB4. All of these events are typical of air-mucosa inflammatory disease. These findings scientifically evidence for the first time the ethnopharmacological use of M. longiflora to treat chronic inflammatory events, such as asthma, and suggest a potential therapeutic use or complementary therapy for this plant extract. [Display omitted]