Phenotypic and genetic diversity among rhizobia isolated from three Hedysarum species: H. spinosissimum, H. coronarium and H. flexuosum

Cultural, physiological and biochemical properties of 18 strains of rhizobia isolated from root nodules of the forage legume H. spinosissimum were compared with those of rhizobia from the related species H. coronarium (15 strains) and H. flexuosum (four strains). On the basis of 43 characteristics t...

Full description

Saved in:
Bibliographic Details
Published in:Plant and soil 2003-04, Vol.251 (1), p.143-153
Main Authors: Kishinevsky, B. D., Nandasena, K. G., Yates, R. J., Nemas, C., Howieson, J.G.
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Bradyrhizobium
Fundamental and applied biological sciences. Psychology
Genetic diversity
Legumes
Mesorhizobium
Microbiology
Nitrogen
Nitrogen fixation
Nodulation
Numerical analysis
Phosphates
Plants
Rhizobium
Rhizobium leguminosarum
rRNA genes
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cultural, physiological and biochemical properties of 18 strains of rhizobia isolated from root nodules of the forage legume H. spinosissimum were compared with those of rhizobia from the related species H. coronarium (15 strains) and H. flexuosum (four strains). On the basis of 43 characteristics the 37 strains of Hedysarum rhizobia could be divided into two groups by numerical analysis. The H. spinosissimum rhizobia formed the first group and the second group comprised the strains from H. coronarium and H. flexuosum. The reference Rhizobium leguminosarum bv. viceae strain 250A was clustered with the rhizobia from H. coronarium and H. flexuosum. By contrast Bradyrhizobium sp. (Arachis) reference strain 280A was not clustered with any of the strains tested, indicating that the H. spinosissimum rhizobia differ from both Rhizobium and Bradyrhizobium. Serological data also discriminate between H. spinosissimum and H. coronarium rhizobia but not between the latter and H. flexuosum strains. The strains tested exhibit a high degree of specificity for nodulation and nitrogen fixation. We also determined the 16SrRNA gene sequence of H. spinosissimum rhizobia (four strains), H. coronarium (two strains) and H. flexuosum (two strains) and found that the four H. spinosissimum isolates share a 98% identity among each other in this region but they showed less than 92% identity to the H. coronarium and H. flexuosum isolates. The H. spinosissimum isolates were closely related to both Mesorhizobium loti and M. ciceri, sharing 97% identity with each species.
ISSN:0032-079X
1573-5036
DOI:10.1023/A:1022967213088