Optimization of Cellulase Production by Halobacillus sp. QLS 31 Isolated from Lake Qarun, Egypt

A halophilic cellulase-producing bacterium was isolated from a sediment sample collected from Lake Qarun (Fayoum Province, Egypt). Molecular identification based on 16S rDNA amplification and sequencing revealed 99% homology with Halobacillus sp. and hence was designated as Halobacillus sp. QLS 31....

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2017-09, Vol.183 (1), p.189-199
Main Authors: Korany, Ahmed H., Ali, Amal E., Essam, Tamer M., Megahed, Salwa A.
Format: Article
Language:English
Subjects:
Ammonium
Ammonium sulfate
Bacteria
Biochemistry
Biotechnology
Calcium
Carboxymethyl cellulose
Carboxymethylcellulose
Cell culture
Cellulase
Cellulose
Chemistry
Chemistry and Materials Science
Enzymes
Fermentation
Homology
Inoculum
Lakes
Magnesium
Metal concentrations
Optimization
pH effects
rRNA 16S
Sediment samplers
Sodium chloride
Sulfates
Yeast
Yeasts
Zinc
Zinc sulfate
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Summary:A halophilic cellulase-producing bacterium was isolated from a sediment sample collected from Lake Qarun (Fayoum Province, Egypt). Molecular identification based on 16S rDNA amplification and sequencing revealed 99% homology with Halobacillus sp. and hence was designated as Halobacillus sp. QLS 31. Medium composition and culture conditions were optimized for enhancing the production of cellulase enzyme using the Plackett-Burman statistical design. Ten variables were evaluated for their influence on cellulase production. Carboxymethyl cellulose (CMC), zinc sulfate (ZnSO 4 ), and inoculum size were found to exert a significant effect on cellulase productivity by Halobacillus sp. QLS 31. The maximum specific activity of cellulase enzyme was 48.08 U/mg. Following the predicted conditions, a 7.5-fold increase in cellulase specific activity (175.47 U/mg) was achieved compared to the basal medium (23.19 U/mg) under the following optimized conditions: temperature (30 °C), fermentation time (2 days ), pH value (9), CMC concentration (1%), inoculum size (1%), yeast extract concentration (0.1%), ammonium sulfate ((NH 3 ) 2 SO 4 ) concentration (0.1%), sodium chloride (NaCl) concentration (20%), and metal inducers: ZnSO 4 (0.1%) and Ca/Mg ratio (0.01%). Thus, the results of this study provide an important basis for more efficient, cheap industrial cellulase production from halophilic Halobacillus sp. QLS 31.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-017-2438-z