Unambiguous high resolution genotyping of human leukocyte antigens

We have developed a high resolution sequencing based typing method for genotyping Human Leukocyte Antigens (HLA) over a period of twenty years. The methods are based upon the separation of HLA alleles per locus at the initial amplification to simplify the analysis post-sequencing. The increasing dis...

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Bibliographic Details
Published in:Journal of immunological methods 2017-06, Vol.445, p.10-14
Main Authors: Bradshaw, Robert A., Dunn, Paul P.J.
Format: Article
Language:English
Subjects:
Amplification and sequencing primers
Genotype
High resolution unambiguous HLA typing
HLA Antigens - genetics
HLA Antigens - immunology
HLA class I (HLA-A, B, C) genotyping
HLA class II (DRB1, DQB1) genotyping
Humans
Sequence Analysis, DNA
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Description
Summary:We have developed a high resolution sequencing based typing method for genotyping Human Leukocyte Antigens (HLA) over a period of twenty years. The methods are based upon the separation of HLA alleles per locus at the initial amplification to simplify the analysis post-sequencing. The increasing discovery of polymorphism in HLA, manifested in new alleles, has necessitated the continuing development of this method. Here we present methods for the high resolution Sequence Based Typing of HLA-A, B, C (class I) and HLA-DQB1 and DRB1 (class II). The purpose of this article is to provide a valuable resource of methods and primers for other laboratories engaged in HLA typing.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2017.02.011