Combined effect of pulsed electromagnetic field and sound wave on In vitro and In vivo neural differentiation of human mesenchymal stem cells

• Published in: Biotechnology progress 2017-01, Vol.33 (1), p.201-211
• Main Authors: Choi, Yun‐Kyong, Urnukhsaikhan, Enerelt, Yoon, Hee‐Hoon, Seo, Young‐Kwon, Cho, Hyunjin, Jeong, Jong‐Seob, Kim, Soo‐Chan, Park, Jung‐Keug
• Format: Article
• Language: English
• Subjects: Bone Marrow Cells - cytology; Cell Differentiation - radiation effects; Cell Proliferation - radiation effects; combined stimulus; Electromagnetic Fields; Gene Expression Regulation, Developmental - radiation effects; human bone marrow mesenchymal stem cells; Humans; Mesenchymal Stromal Cells - radiation effects; neural differentiation; Neural Stem Cells - radiation effects; Neurons - cytology; Neurons - radiation effects; Osteogenesis - radiation effects; pulsed electromagnetic fields; Sound; sound wave
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1002/btpr.2389

Biophysical wave stimulus has been used as an effective tool to promote cellular maturation and differentiation in the construction of engineered tissue. Pulsed electromagnetic fields (PEMFs) and sound waves have been selected as effective stimuli that can promote neural differentiation. The aim of this study was to investigate the synergistic effect of PEMFs and sound waves on the neural differentiation potential in vitro and in vivo using human bone marrow mesenchymal stem cells (hBM–MSCs). In vitro, neural‐related genes in hBM–MSCs were accelerated by the combined exposure to both waves more than by individual exposure to PEMFs or sound waves. The combined wave also up‐regulated the expression of neural and synaptic‐related proteins in a three‐dimensional (3‐D) culture system through the phosphorylation of extracellular signal‐related kinase. In a mouse model of photochemically induced ischemia, exposure to the combined wave reduced the infarction volume and improved post‐injury behavioral activity. These results indicate that a combined stimulus of biophysical waves, PEMFs and sound can enhance and possibly affect the differentiation of MSCs into neural cells. Our study is meaningful for highlighting the potential of combined wave for neurogenic effects and providing new therapeutic approaches for neural cell therapy. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:201–211, 2017