Effect of temperature on plasmid stability and expression of cloned cellulase gene in a recombinantBacillus megaterium

The expression of carboxymethyl cellulase gene inBacillus megaterium (pCK108) was investigated with respect to temperature in batch culture. The suboptimal temperature supporting maximal cell growth rate was determined to be 30 °C at which stability of the plasmid pCK108 could be maintained stable....

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 1987-12, Vol.9 (12), p.821-824
Main Authors: Son, Kwang H, Jang, Jong H, Kim, Jung H
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The expression of carboxymethyl cellulase gene inBacillus megaterium (pCK108) was investigated with respect to temperature in batch culture. The suboptimal temperature supporting maximal cell growth rate was determined to be 30 °C at which stability of the plasmid pCK108 could be maintained stable. However, cellular plasmid contents, production rate of cellulase of the cell, and efficiency of the gene expression increased significantly with increase of the temperature from 30 °C to 44 °C, even though the plasmid stability decreased up to 60% level at the end of the culture.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01026189