Copper resistance in Desulfovibrio strain R2

A sulfate-reducing bacterium, designated as strain R2, was isolated from wastewater of a ball-bearing manufacturing facility in Tomsk, Western Siberia. This isolate was resistant up to 800 mg Cu/l in the growth medium. By comparison, Cu-resistance of reference cultures of sulfate-reducing bacteria r...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2003, Vol.83 (1), p.99-106
Main Authors: Karnachuk, Olia V, Kurochkina, Svetlana Y, Nicomrat, Duongruitai, Frank, Yulia A, Ivasenko, Denis A, Phyllipenko, Elena A, Tuovinen, Olli H
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - genetics
Bioremediation
Copper
Copper - pharmacology
Culture Media
Desulfovibrio - classification
Desulfovibrio - drug effects
Desulfovibrio - genetics
DNA, Ribosomal - analysis
Drug Resistance, Bacterial - genetics
Industrial Waste
Manufacturing industry
Metallurgy
Microbiology
Molecular Sequence Data
Oxidation-Reduction
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Sulfate reduction
Sulfates
Sulfates - metabolism
Trace elements
Water Microbiology
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Summary:A sulfate-reducing bacterium, designated as strain R2, was isolated from wastewater of a ball-bearing manufacturing facility in Tomsk, Western Siberia. This isolate was resistant up to 800 mg Cu/l in the growth medium. By comparison, Cu-resistance of reference cultures of sulfate-reducing bacteria ranged from 50 to 75 mg Cu/l. Growth experiments with strain R2 showed that Cu was an essential trace element and, on one hand, enhanced growth at concentrations up to 10 mg/l but, on the other hand, the growth rate decreased and lag-period extended at copper concentrations of >50 mg/l. Phenotypic characteristics and a 1078 bp nucleotide sequence of the 16S rDNA placed strain R2 within the genus Desulfovibrio. Desulfovibrio R2 carried at least one plasmid of approximately of 23.1 kbp. A 636 bp fragment of the pcoR gene of the pco operon that encodes Cu resistance was amplified by PCR from plasmid DNA of strain R2. The pco genes are involved in Cu-resistance in some enteric and aerobic soil bacteria. Desulfovibrio R2 is a prospective strain for bioremediation purposes and for developing a homologous system for transformation of Cu-resistance in sulfate-reducing bacteria.
ISSN:0003-6072
1572-9699
DOI:10.1023/A:1022947302637