Protocol for rapid fluorescence in situ hybridization of bacteria in cryosections of microarthropods

A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2003-05, Vol.69 (5), p.2875-2878
Main Authors: Thimm, T, Tebbe, C.C
Format: Article
Language:English
Subjects:
Animals
Arthropods
Arthropods - microbiology
Bacteria
Bacteria - genetics
Bacteria - isolation & purification
Base Sequence
Biological and medical sciences
Digestive System - microbiology
DNA probes
DNA Probes - genetics
Fluorescence
fluorescence in situ hybridization
fluorescence microscopy
Folsomia candida
Frozen Sections
Fundamental and applied biological sciences. Psychology
genes
Hybridization
In Situ Hybridization, Fluorescence - methods
intestinal microorganisms
Methods
Microbiology
microscopy
Microscopy, Fluorescence - methods
midgut
ribosomal RNA
soil insects
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Summary:A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and reduces the number of manipulations which might damage the microscopic material. The method allowed the study of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria in both the gut and tissue.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.69.5.2875-2878.2003