Effect of sodium selenite on isoflavonoid contents and antioxidant capacity of chickpea (Cicer arietinum L.) sprouts

•Germination using sodium selenite increased chickpea isoflavonoids’ concentration.•Formononetin and its malonylated glycoside increased in selenized sprouts.•Selenized chickpea sprouts showed higher antioxidant capacity than controls. Isoflavonoid compositions, phenylalanine ammonia lyase (PAL) act...

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Bibliographic Details
Published in:Food chemistry 2017-07, Vol.226, p.69-74
Main Authors: Guardado-Félix, Daniela, Serna-Saldivar, Sergio O., Cuevas-Rodríguez, Edith O., Jacobo-Velázquez, Daniel A., Gutiérrez-Uribe, Janet A.
Format: Article
Language:English
Subjects:
Antioxidants
Cicer - chemistry
Cicer - drug effects
Cicer - enzymology
Cicer arietinum L
Gene Expression Regulation, Plant
Germination
Isoflavones
Isoflavonoids
Phenylalanine Ammonia-Lyase - genetics
Seedlings - chemistry
Seedlings - drug effects
Seedlings - enzymology
Selenium
Sesquiterpenes
Sodium Selenite - pharmacology
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Summary:•Germination using sodium selenite increased chickpea isoflavonoids’ concentration.•Formononetin and its malonylated glycoside increased in selenized sprouts.•Selenized chickpea sprouts showed higher antioxidant capacity than controls. Isoflavonoid compositions, phenylalanine ammonia lyase (PAL) activity and antioxidant capacity were evaluated in chickpea (Cicer arietinum L.) sprouts germinated after soaking with different sodium selenite (Na2SeO3) concentrations (0, 1 and 2mg/100g seeds). Chickpea seeds were germinated during four days at 24°C and the isoflavonoid profiles and concentrations evaluated by HPLC-UV daily during four days of germination. Eleven isoflavones and two pterocarpan phytoalexins forms were identified in sprouts, being malonylated formononetin glycoside, formononetin, isoformononetin glycoside and malonylated biochanin A glycoside the major compounds. Compared to untreated sprouts, total isoflavonoid, PAL activity and antioxidant capacity showed a remarkable increase of 83%, 56%, and 33%, respectively in chickpea sprouts that were treated with a high sodium selenite content (2mg/100g seeds). Results suggest that Se-enriched chickpea sprouts could represent a good source of dietary Se and as an upgraded source of isoflavonoids.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2017.01.046